Subcutaneous tissue fibroblast cytoskeletal remodeling induced by acupuncture: Evidence for a mechanotransduction-based mechanism
Subcutaneous tissue fibroblast cytoskeletal remodeling induced by acupuncture: Evidence for a mechanotransduction-based mechanism
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DOI：
10.1002/jcp.20623
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发表时间：
2006-06-01
影响因子：
5.6
通讯作者：
Howe, AK
中科院分区：
文献类型：
--
作者：
Langevin, HM;Bouffard, NA;Howe, AK
针灸针旋转先前已被证明会引起皮下结缔组织的特定机械刺激。本研究使用针灸来研究基于机械转导的机制在机械诱导的细胞骨架重塑中的作用。通过共聚焦显微镜成像的小鼠组织外植体的形态测量分析来量化针灸针旋转的效果。针旋转在30分钟内诱导广泛的成纤维细胞伸展和片状伪足形成，可以通过细胞体横截面积的增加来测量。旋转的影响达到峰值与两个针旋转，并随着旋转的进一步增加而下降。整个组织中存在显著的旋转效应，表明存在横向延伸超过几厘米的响应。通过肌动球蛋白收缩性（blebbistatin）、Rho激酶（Y-27632和H-1152）和Rac信号传导的药理学抑制剂来防止两次针旋转的效果。在这项研究中所展示的成纤维细胞的积极的细胞骨架反应构成了重要的一步，在整个组织中理解细胞的机械力转导响应外部施加的机械刺激，并支持先前提出的模型，涉及结缔组织机械力转导的针灸机制。
Acupuncture needle rotation has been previously shown to cause specific mechanical stimulation of subcutaneous connective tissue. This Study uses acupuncture to investigate the role of mechanotransduction-based mechanisms in mechanically-induced cytoskeletal remodeling. The effect of acupuncture needle rotation was quantified by morphometric analysis Of mouse tissue explants imaged with confocal microscopy. Needle rotation induced extensive fibroblast spreading and lamellipodia formation within 30 min, measurable as an increased in cell body cross sectional area. The effect of rotation peaked with two needle revolutions and decreased with further increases in rotation. Significant effects of rotation were present throughout the tissue, indicating the presence of a response extending laterally over several centimeters. The effect of rotation with two needle revolutions was prevented by pharmacological inhibitors of actomyosin contractility (blebbistatin), Rho kinase(Y-27632 and H-1152), and Rac signaling. The active cytoskeletal response of fibroblasts demonstrated in this Study constitutes an important step in understanding cellular mechanotransduction responses to externally applied mechanical stimuli in whole tissue, and supports a previously proposed model for the mechanism of acupuncture involving connective tissue mechanotransduction.