LYSOSOMAL ACID-PHOSPHATASE IS TRANSPORTED TO LYSOSOMES VIA THE CELL-SURFACE
LYSOSOMAL ACID-PHOSPHATASE IS TRANSPORTED TO LYSOSOMES VIA THE CELL-SURFACE
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DOI：
10.1002/j.1460-2075.1989.tb08537.x
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发表时间：
1989-12-01
期刊：
影响因子：
11.4
通讯作者：
VONFIGURA, K
中科院分区：
文献类型：
--
作者：
BRAUN, M;WAHEED, A;VONFIGURA, K
溶酶体酸性磷酸酶（Lysosomal acid phosphatase，ALP）作为一种跨膜蛋白被转运到致密的溶酶体中。溶酶体的途径包括通过质膜。LAP从反式高尔基体转运至细胞表面，半衰期< 10分钟。细胞表面LAP迅速内化。大部分内化的蛋白质被转运回细胞表面。平均而言，每个β-内酰胺分子在转移到致密溶酶体之前在细胞表面和内体之间循环> 15次。在平衡状态下内体中存在的α-淀粉酶前体是细胞表面的4倍。将细胞暴露于低温的弱碱如NH_4Cl、氯喹和伯氨喹，降低了细胞表面的稳态浓度。再循环路径在≥20.degree.不包括高尔基体/反高尔基体网络的通过。在5-6小时的半衰期内，将β-内酰胺从质膜/内体池转移到致密溶酶体，从那里它不会以可测量的速率再循环到内体/质膜池。
Lysosomal acid phosphatase (LAP) is transported as a transmembrane protein to dense lysosomes. The pathway of LAP to lysosomes includes the passage through the plasma membrane. LAP is transported from the trans-Golgi to the cell surface with a half-time of < 10 min. Cell surface LAP is rapidly internalized. Most of the internalized LAP is transported back to the cell surface. On average, each LAP molecule cycles > 15 times between the cell surface and the endosomes before it is transferred to dense lysosomes. At equilibrium .apprx. 4 times more LAP precursor is present in endosomes than at the cell surface. Exposing cells to reduced temperature of weak basis such as NH4Cl, chloroquine and primaquine decreases the steady-state concentration of LAP at the cell surface. The recycling pathway is operative at .gtoreq. 20.degree. C and does not include passage of the Golgi/trans-Golgi network. LAP is transferred with a half-time of 5-6 h from the plasma membrane/endosome pool to dense lysosomes, from where it does not recycle to the endosome/plasma membrane pool at a measurable rate.