Increased expression in vivo of VCAM-1 and E-selectin by the aortic endothelium of normolipemic and hyperlipemic diabetic rabbits.
Increased expression in vivo of VCAM-1 and E-selectin by the aortic endothelium of normolipemic and hyperlipemic diabetic rabbits.
复制标题
正常血脂和高血脂糖尿病兔的主动脉内皮体内 VCAM-1 和 E-选择素的表达增加。
DOI：
10.1161/01.atv.14.5.760
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Cybulsky,MI
中科院分区：
文献类型：
--
作者：
Richardson,M;Hadcock,SJ;DeReske,M;Cybulsky,MI
动脉粥样硬化在糖尿病患者中增强，但涉及的机制仍不清楚。白细胞-内皮细胞相互作用的增加可能参与其中，因为单核白细胞粘附于内皮细胞是实验性动脉粥样硬化和四氧嘧啶诱导的兔糖尿病的早期事件。在en face Häutchen内皮制备物中使用原位免疫组织化学来鉴定用内皮白细胞粘附分子（血管细胞粘附分子-1 [VCAM-1]和E-选择素）抗体染色的内皮细胞，并确定每10，000个细胞中的染色细胞数。将正常血脂和蛋黄饮食诱导的糖尿病兔的血糖制剂与类似饮食的血糖正常动物的血糖制剂进行比较。血管壁的横截面用油红O和VCAM-1、E-选择素和RAM-11阳性巨噬细胞的抗体染色。4周后，VCAM-1或E-选择素的细胞表达的频率显着增加，与正常血脂对照组相比，这种频率进一步增加，在糖尿病兔动脉粥样硬化。VCAM-1和E-选择素的表达在正常血脂的糖尿病兔血管中比在高血脂、正常血糖的血管中更频繁。这些粘附分子在糖尿病动物中表达的增强可能为与糖尿病相关的动脉粥样硬化增强提供部分解释。
Atherosclerosis is enhanced in humans with diabetes mellitus, but the mechanism(s) involved remains unclear. Increased leukocyte-endothelium interaction may be involved, since mononuclear leukocyte adherence to the endothelium is an early event in both experimental atherosclerosis and alloxan-induced diabetes in rabbits. In situ immunohistochemistry was used in en face Häutchen endothelial preparations to identify endothelial cells that stained with antibodies to endothelial leukocyte adhesion molecules (vascular cell adhesion molecule-1 [VCAM-1] and E-selectin), and the number of stained cells per 10,000 cells was determined. Preparations from aortas of diabetic normolipemic and egg yolk diet-induced hyperlipemic diabetic rabbits were compared with those from normoglycemic animals on similar diets. Cross sections of the vessel wall were stained with oil red O and antibodies to VCAM-1, E-selectin, and RAM-11-positive macrophages. After 4 weeks of hyperlipemia the frequency of cells expressing VCAM-1 or E-selectin was significantly increased compared with normolipemic controls; this frequency was further increased in the aortas of hyperlipemic diabetic rabbits. VCAM-1 and E-selectin expression was more frequent in normolipemic diabetic rabbit aortas than in hyperlipemic, normoglycemic vessels. The potentiation of expression of these adhesion molecules in diabetic animals may provide part of the explanation for the enhanced atherosclerosis associated with diabetes mellitus.