Alteration of fibrin network by activated protein C.
Alteration of fibrin network by activated protein C.
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DOI：
10.1182/blood.v83.9.2541.2541
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发表时间：
1994-05
期刊：
影响因子：
20.3
通讯作者：
A. Gruber;E. Mori;G. D. del Zoppo;L. Waxman;J. Griffin
中科院分区：
文献类型：
--
作者：
A. Gruber;E. Mori;G. D. del Zoppo;L. Waxman;J. Griffin
抗血栓血浆酶，活化蛋白C（APC），可能在溶栓中发挥作用。在体外，APC加速凝块溶解取决于其抑制凝血酶原活化的能力。采用比浊法、纤溶酶消化纤维蛋白和电子显微镜观察血浆凝块，研究APC对纤维蛋白网络组装和分散的影响。在凝血前而不是凝血后加入APC加速了凝块溶解。APC可降低凝血血浆浊度的增加率。含APC的血浆凝块的浊度与凝块溶解时间直接相关。来自在APC存在下形成的血浆凝块的纤维蛋白比来自不添加APC的凝块的纤维蛋白产生更少的纤维蛋白降解产物。此外，APC减少了凝胶组装过程中血浆凝块中纤维蛋白纤维的直径和相对数量。我们认为APC可能通过减少成熟血栓内纤维蛋白的相对质量来增强溶栓的功效。
The antithrombotic plasma enzyme, activated protein C (APC), may play a role in thrombolysis. In vitro, acceleration of clot lysis by APC depends on its ability to inhibit the activation of prothrombin. The effect of APC on the assembly and dispersion of fibrin network was studied using turbidimetry, plasmin digestion of fibrin, and electron microscopy of plasma clots. The addition of APC before clotting but not after clotting accelerated clot lysis. The rate of increase in the turbidity of clotting plasma was reduced by APC. The turbidity of plasma clots containing APC was directly related to the clot lysis time. Fibrin from plasma clots that were formed in the presence of APC yielded less fibrin degradation products than fibrin from clots without added APC. Furthermore, APC reduced the diameter and relative number of fibrin fibers in plasma clots during gel assembly. We propose that APC may enhance the efficacy of thrombolysis by reducing the relative mass of fibrin within maturing thrombi.