Genome-wide transcriptional profiling of human glioblastoma cells in response to ITE treatment.
Genome-wide transcriptional profiling of human glioblastoma cells in response to ITE treatment.
复制标题
人类胶质母细胞瘤细胞响应 ITE 治疗的全基因组转录谱分析
DOI：
10.1016/j.gdata.2015.06.025
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发表时间：
2015-09
期刊：
影响因子：
--
通讯作者：
Wang YJ
中科院分区：
文献类型：
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作者：
Kang B;Zhou Y;Zheng M;Wang YJ
配体激活的转录因子芳烃受体（AhR）最近被发现在胚胎发生和肿瘤发生中发挥关键作用（Feng等，Safe等），2-（1 ' h -吲哚-3 ' -羰基）-噻唑-4-羧酸甲酯（ITE） （Song等）是一种内源性AhR配体，具有抗肿瘤活性。为了深入了解ITE如何通过AhR在胚胎发生和肿瘤发生中起作用，我们分析了以下三组细胞的全基因组转录谱：人胶质母细胞瘤U87亲本细胞、用载体（DMSO）处理的U87肿瘤球细胞和用ITE处理的U87肿瘤球细胞。在这里，我们提供了样本收集策略的细节，并展示了与我们的基因阵列数据相关的质量控制和分析，这些数据存储在基因表达Omnibus （GEO）中，登录代码为GSE67986。
A ligand-activated transcription factor aryl hydrocarbon receptor (AhR) is recently revealed to play a key role in embryogenesis and tumorigenesis (Feng et al., Safe et al.) and 2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE) (Song et al.) is an endogenous AhR ligand that possesses anti-tumor activity. In order to gain insights into how ITE acts via the AhR in embryogenesis and tumorigenesis, we analyzed the genome-wide transcriptional profiles of the following three groups of cells: the human glioblastoma U87 parental cells, U87 tumor sphere cells treated with vehicle (DMSO) and U87 tumor sphere cells treated with ITE. Here, we provide the details of the sample gathering strategy and show the quality controls and the analyses associated with our gene array data deposited into the Gene Expression Omnibus (GEO) under the accession code of GSE67986.