Seasonal incidence and molecular characterization of Salmonella from dairy cows, calves, and farm environment
Seasonal incidence and molecular characterization of Salmonella from dairy cows, calves, and farm environment
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DOI：
10.1089/fpd.2007.0048
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发表时间：
2008-02-01
影响因子：
2.8
通讯作者：
Draughon, F. A.
中科院分区：
文献类型：
--
作者：
Pangloli, Philipus;Dje, Yobouet;Draughon, F. A.
沙门氏菌的发生模式和分子特征对于病原体的监测和控制具有重要意义。本研究的目的是确定奶牛动物和环境中沙门氏菌发生的逐月变化和季节性影响，并表征选定的沙门氏菌分离株。在一项为期 12 个月的研究中，对每月从奶牛场收集的总共 7680 个动物和环境样本进行了沙门氏菌的分析。奶牛场沙门氏菌的主要来源（阳性百分比）是冬季挤奶厅空气（62%）和鸟粪（63%）；春季饲料（50-58%）、水（53-67%）、犊牛垫料（63%）、土壤（60-63%）、挤奶厅空气（60%）和鸟粪（50%）；除挤奶厅空气 (25%) 和散装罐外的所有动物和环境样本 (40-92%)。夏季牛奶（29%）；秋季期间的饲料（60-71%）、牛垫料（59%）、牛土壤（50%）、空气（46-71%）和昆虫（63%）。沙门氏菌核分型表明，大多数血清型来自不同来源，但有些可能源自共同来源，并在农场的不同地点传播。这些数据提供了有关启动农场管理计划以控制这种重要食源性病原体所需的关键动物和环境采样点的一些重要信息。
The occurrence patterns and molecular characteristics of Salmonella are important for surveillance and control of the pathogens. Objectives of this study were to determine month-to-month variation and seasonal effects on the occurrence of Salmonella in dairy animals and environments and to characterize selected Salmonella isolates. A total of 7680 animal and environmental samples, collected monthly from a dairy farm, were analyzed for the presence of Salmonella during a 12-month study. Major sources of Salmonella on the dairy farm (% positive) were milking parlor air (62%) and bird droppings (63%) during winter; feeds (50-58%), water (53-67%), calf bedding (63%), soils (60-63%), milking parlor air (60%), and bird droppings (50%) in spring; all animal and environmental samples (40-92%) except milking parlor air (25%) and bulk tank. milk (29%) in summer; and feeds (60-71%), cow beddings (59%), cow soils (50%), air (46-71%), and insects (63%) during fall. Salmonella ribotyping indicated that most serovars came from different sources but some might have originated from a common source and transmitted from site to site on the farm. These data provide some important information on key animal and environmental sampling sites needed to initiate on-farm management programs for control of this important foodborne pathogen.