Mast cells migrate from blood to brain
Mast cells migrate from blood to brain
复制标题
DOI：
10.1523/jneurosci.20-01-00401.2000
复制
发表时间：
2000-01-01
影响因子：
5.3
通讯作者：
Silver, R
中科院分区：
文献类型：
--
作者：
Silverman, AJ;Sutherland, AK;Silver, R
肥大细胞（MCs）存在于许多物种的中枢神经系统中，这一点已经得到了很好的证实。此外，由于生理条件的改变，它们的数量在成年后会迅速增加。在这项研究中，我们发现产后早期的大鼠在丘脑中的肥大细胞明显多于未生育的对照组。来自这些女性的半薄切片的证据表明，肥大细胞正在穿过中等大小的血管。我们假设肥大细胞数量的增加是由它们迁移到神经实质引起的。为此，我们纯化大鼠腹膜肥大细胞，用重要染料PKH26或CellTracker Green标记，并注射到宿主动物体内。注射后1小时，将含有组胺或血清素（储存在肥大细胞中的介质）的染色细胞放置在丘脑血管附近。注射细胞约占该脑区肥大细胞总数的2-20%。扫描共聚焦显微镜证实，生物胺和重要染料发生在同一个细胞中。为了确定供体肥大细胞是否在血脑屏障内，我们研究了染料标记的供体细胞和因子VIII（内皮基层的一种成分）或胶质纤维酸性蛋白（星形胶质细胞中发现的中间纤维）的定位。共聚焦图像的连续切片重建显示，肥大细胞深至基底层，位于胶质突巢中。这是第一次证明肥大细胞可以快速穿透脑血管，这可能是生理操作后肥大细胞数量快速增加的原因。
It is well established that mast cells (MCs) occur within the CNS of many species. Furthermore, their numbers can increase rapidly in adults in response to altered physiological conditions. In this study we found that early postpartum rats had significantly more mast cells in the thalamus than virgin controls. Evidence from semithin sections from these females suggested that mast cells were transiting across the medium-sized blood vessels. We hypothesized that the increases in mast cell number were caused by their migration into the neural parenchyma. To this end, we purified rat peritoneal mast cells, labeled them with the vital dyes PKH26 or CellTracker Green, and injected them into host animals. One hour after injection, dye-filled cells, containing either histamine or serotonin (mediators stored in mast cells), were located close to thalamic blood vessels. Injected cells represented similar to 2-20% of the total mast cell population in this brain region. Scanning confocal microscopy confirmed that the biogenic amine and the vital dye occurred in the same cell. To determine whether the donor mast cells were within the blood-brain barrier, we studied the localization of dye-marked donor cells and either Factor VIII, a component of endothelial basal laminae, or glial fibrillary acidic protein, the intermediate filament found in astrocytes. Serial section reconstructions of confocal images demonstrated that the mast cells were deep to the basal lamina, in nests of glial processes. This is the first demonstration that mast cells can rapidly penetrate brain blood vessels, and this may account for the rapid increases in mast cell populations after physiological manipulations.