A naphthalimide based fast and selective fluorescent probe for hypochlorous acid/hypochlorite and its application for bioimaging
A naphthalimide based fast and selective fluorescent probe for hypochlorous acid/hypochlorite and its application for bioimaging
复制标题
基于萘酰亚胺的次氯酸/次氯酸盐快速选择性荧光探针及其在生物成像中的应用
DOI：
10.1039/c8nj03208d
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发表时间：
2018-09-21
影响因子：
3.3
通讯作者：
Guo, Wei
中科院分区：
文献类型：
--
作者：
Lv, Xin;Yuan, Xia;Guo, Wei
设计并合成了一种含1，8-萘酰亚胺和硒代吗啉片段的可逆线粒体靶向荧光探针（Nap-Se）。该探针在磷酸盐缓冲溶液中对次氯酸/次氯酸盐具有高选择性、灵敏度和快速响应（<2s）。细胞成像研究证实，探针Nap-Se特异性定位于活细胞的线粒体中，可用于监测RAW 264.7细胞中内源性和外源性HClO/ClO−的水平。
A reversible and mitochondria targetable fluorescent probe (Nap-Se) bearing 1,8-naphthalimide and a selenomorpholine fragment was designed and synthesized. The probe showed a highly selective, sensitive and fast response (<2 s) toward hypochlorous acid/hypochlorite in phosphate buffer saline solution. Cell imaging studies confirmed that probe Nap-Se was specifically localized in mitochondria of living cells and could be used to monitor the levels of endogenous and exogenous HClO/ClO− in RAW264.7 cells.