Potential markers of tongue tumor progression selected by cDNA microarray
Potential markers of tongue tumor progression selected by cDNA microarray
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DOI：
10.1177/039463200501800311
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发表时间：
2005-07-01
影响因子：
3.5
通讯作者：
Pezzetti, F
中科院分区：
文献类型：
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作者：
Carinci, F;Lo Muzio, L;Pezzetti, F
鳞状细胞癌（SCC）是口腔最常见的恶性肿瘤，通常表现为预后差，转移是主要的死亡原因。这种肿瘤通常起源于恶性前病变。迄今为止，很难预测是否和哪些恶性前病变可能发展为口腔鳞状细胞癌使用传统方法。由于这些原因，一些研究正试图确定在恶性病变和肿瘤进展中有用的标志物。为了确定舌肿瘤进展的基因表达谱，我们使用含有19.2个K克隆的cDNA芯片，比较了9例发育不良（DS）、8例无转移性肿瘤（TWM）、11例转移性SCCs （MT）和11例正常组织的基线。我们最初基于所有6026个克隆的信息应用了分层聚集聚类。结果通过执行两步分析获得：微阵列的显著性分析（SAM）和基因本体搜索。105个克隆的表达水平有统计学上的显著差异（FDR）
Squamous cell carcinoma (SCC), the most frequent malignant tumor of the oral cavity, generally exhibits a poor prognosis and metastases are the main cause of death. This tumor often arises from pre-malignant lesions. To date, it is difficult to predict if and which pre-malignant lesions may progress into oral SCC using traditional methods. For these reasons, several studies are trying to identify markers useful in the progression of pre-malignant lesions and tumors. To define the genetic expression profile of tongue tumor progression we compared 9 dysplasias (DS), 8 tumors without metastasis (TWM), 11 metastasizing SCCs (MT) of the tongue, and a baseline of 11 normal tissues by using cDNA microarray containing 19.2 K clones. We initially applied hierarchical agglomerative clustering based on information from all 6026 clones. Results were obtained by performing a two steps analysis: a Significance Analysis of Microarray (SAM) and a Gene Ontology search. One hundred and five clones have statistically significant different expression levels (FDR