Surface plasmon resonance scattering and absorption of anti-EGFR antibody conjugated gold nanoparticles in cancer diagnostics: Applications in oral cancer
Surface plasmon resonance scattering and absorption of anti-EGFR antibody conjugated gold nanoparticles in cancer diagnostics: Applications in oral cancer
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DOI：
10.1021/nl050074e
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发表时间：
2005-05-01
期刊：
影响因子：
10.8
通讯作者：
El-Sayed, MA
中科院分区：
文献类型：
--
作者：
El-Sayed, IH;Huang, XH;El-Sayed, MA
金纳米粒子具有独特的光学特性，可作为活体细胞中的生物传感器。利用一种简单而廉价的技术，我们记录了胶体金纳米粒子和与单克隆抗表皮生长因子受体（抗egfr）抗体结合的金纳米粒子在非恶性上皮细胞系（HaCaT）和两种恶性口腔上皮细胞系（HOC 313克隆8和HSC 3）的细胞培养物中培养后的表面等离子体共振（SPR）散射图像和SPR吸收光谱。胶体金纳米颗粒在细胞质中以分散和聚集的形式存在，并提供解剖标记信息，但它们的摄取对恶性细胞是非特异性的。抗egfr抗体结合的纳米颗粒特异且均匀地结合在癌型细胞表面，其亲和力比非癌型细胞高600%。与加入到非癌细胞中观察到的相比，这种特异性和均匀的结合发现具有相对更清晰的SPR吸收带，其最大红移。这些结果表明，抗体共轭金纳米颗粒产生的SPR散射成像或SPR吸收光谱可用于体内和体外口腔上皮活癌细胞的分子生物传感器技术诊断和研究。
Gold nanoparticles with unique optical properties may be useful as biosensors in living whole cells. Using a simple and inexpensive technique, we recorded surface plasmon resonance (SPR) scattering images and SPR absorption spectra from both colloidal gold nanoparticles and from gold nanoparticles conjugated to monoclonal anti-epidermal growth factor receptor (anti-EGFR) antibodies after incubation in cell cultures with a nonmalignant epithelial cell line (HaCaT) and two malignant oral epithelial cell lines (HOC 313 clone 8 and HSC 3). Colloidal gold nanoparticles are found in dispersed and aggregated forms within the cell cytoplasm and provide anatomic labeling information, but their uptake is nonspecific for malignant cells. The anti-EGFR antibody conjugated nanoparticles specifically and homogeneously bind to the surface of the cancer type cells with 600% greater affinity than to the noncancerous cells. This specific and homogeneous binding is found to give a relatively sharper SPR absorption band with a red shifted maximum compared to that observed when added to the noncancerous cells. These results suggest that SPR scattering imaging or SPR absorption spectroscopy generated from antibody conjugated gold nanoparticles can be useful in molecular biosensor techniques for the diagnosis and investigation of oral epithelial living cancer cells in vivo and in vitro.