Prominent lectin-like oxidized low density lipoprotein (LDL) receptor-1 (LOX-1) expression in atherosclerotic lesions is associated with tissue factor expression and apoptosis in hypercholesterolemic rabbits
Prominent lectin-like oxidized low density lipoprotein (LDL) receptor-1 (LOX-1) expression in atherosclerotic lesions is associated with tissue factor expression and apoptosis in hypercholesterolemic rabbits
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DOI：
10.1248/bpb.31.1475
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发表时间：
2008-08-01
影响因子：
2
通讯作者：
Saji, Hideo
中科院分区：
文献类型：
--
作者：
Kuge, Yuji;Kume, Noriaki;Saji, Hideo
背景资料：氧化低密度脂蛋白（oxidized low density lipoprotein，LDL）的细胞表面受体-1（lectin like oxidized low density lipoprotein receptor-1，LOX-1）参与动脉粥样硬化的形成和血栓的形成，但LOX-1在动脉粥样硬化斑块不稳定、破裂和血栓形成中的作用尚不清楚。在这里，我们比较了LOX-1的体内表达，与组织因子（TF）的表达和细胞凋亡，在心肌梗死倾向的渡边遗传性高脂血症（WHHLMI）兔动脉粥样硬化病变。方法和结果：我们从四只WHHLMI家兔的主动脉弓和胸主动脉制备了60个系列的横截面。LOX-1和TF的表达，以及凋亡事件分别通过免疫组化染色和TUNEL法测定。LOX-1的表达主要见于易损斑块样动脉粥样硬化病变中富含巨噬细胞的脂质区域，在该区域TF表达和凋亡事件显著。LOX-1表达与TF表达呈正相关（r=0.53，p
Background: Despite increasing in vitro evidence that lectin-like oxidized low density lipoprotein (LDL) receptor-1 (LOX-1), a cell-surface receptor for oxidized LDL, is implicated in the atherogenesis and thrombus formation, its in vivo participation to the atherosclerotic plaque destabilization, rupture and thrombus formation remains unclear. Here, we compared the in vivo expression of LOX-1, with tissue factor (TF) expression and cell apoptosis, in atherosclerotic lesions of myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbits. Methods and Results: We prepared sixty series of cross sections in the aortic arch and the thoracic aorta from four WHHLMI rabbits. LOX-1 and TF expression, as well as apoptotic events were determined by immunohistochemical staining and TUNEL methods, respectively. LOX-1 expression was mainly observed in the macrophage-rich lipid areas of vulnerable plaque-like atheromatous lesions where TF expression and apoptotic events were prominent. LOX-1 expression was positively correlated with TF expression (r=0.53, p