Biophysical studies of bacterial ribosome assembly.
Biophysical studies of bacterial ribosome assembly.
复制标题
细菌核糖体组装的生物物理学研究。
DOI：
10.1016/j.sbi.2008.05.001
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发表时间：
2008
影响因子：
6.8
通讯作者：
Williamson,JamesR
中科院分区：
文献类型：
--
作者：
Williamson,JamesR
细菌核糖体的组装涉及50多种蛋白质与3个大RNA分子的结合，它代表了快速生长的细菌的主要代谢活动。核糖体原子结构的可用性以及生物化学和生物物理方法的应用导致了对核糖体组装机制细节的理解取得了快速进展。概述了组装核糖体所需的基本步骤，并详细介绍了质谱法、计算方法和rna折叠研究在理解这些步骤方面的贡献。这个复杂的过程有顺序和并行的处理，协调以确保有效和完整的核糖体组装，以满足细胞生长的需要。
The assembly of the bacterial ribosome involves the association of over 50 proteins to 3 large RNA molecules, and it represents a major metabolic activity for rapidly growing bacteria. The availability of atomic structures of the ribosome and the application of biochemical and biophysical methods have led to rapid progress in understanding the mechanistic details of ribosome assembly. The basic steps required to assemble a ribosome are outlined, and the contributions of mass spectrometry, computational methods, and RNA-folding studies in understanding these steps are detailed. This complex process takes place with both sequential and parallel processing that is coordinated to ensure efficient and complete assembly of ribosomes to meet the demands of cell growth.