Temporal Variation in Community Composition of Root Associated Endophytic Fungi and Carbon and Nitrogen Stable Isotope Abundance in Two Bletilla Species (Orchidaceae).
Temporal Variation in Community Composition of Root Associated Endophytic Fungi and Carbon and Nitrogen Stable Isotope Abundance in Two Bletilla Species (Orchidaceae).
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两种白芨属（兰科）根相关内生真菌群落组成及碳氮稳定同位素丰度的时间变化
DOI：
10.3390/plants10010018
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发表时间：
2020-12-24
期刊：
影响因子：
--
通讯作者：
Huang W
中科院分区：
文献类型：
--
作者：
Zeng X;Diao H;Ni Z;Shao L;Jiang K;Hu C;Huang Q;Huang W
Mycorrhizae are an important energy source for orchids that may replace or supplement photosynthesis. Most mature orchids rely on mycorrhizae throughout their life cycles. However, little is known about temporal variation in root endophytic fungal diversity and their trophic functions throughout whole growth periods of the orchids. In this study, the community composition of root endophytic fungi and trophic relationships between root endophytic fungi and orchids were investigated in Bletilla striata and B. ochracea at different phenological stages using stable isotope natural abundance analysis combined with molecular identification analysis. We identified 467 OTUs assigned to root-associated fungal endophytes, which belonged to 25 orders in 10 phyla. Most of these OTUs were assigned to saprotroph (143 OTUs), pathotroph-saprotroph (63 OTUs) and pathotroph-saprotroph-symbiotroph (18 OTUs) using FunGuild database. Among these OTUs, about 54 OTUs could be considered as putative species of orchid mycorrhizal fungi (OMF). For both Bletilla species, significant temporal variation was observed in the diversity of root endophytic fungi. The florescence and emergence periods had higher fungal community richness of total species and endemic species than did other periods. Both Bletilla species were dominated by Agaricomycetes and Basidiomycota fungi throughout the whole year; however, their abundances varied between two Bletilla species and among phenological stages. Meanwhile, the ranges of 13C and 15N natural abundance were also highly dynamic across all growth stages of Bletilla species. Compared with the surrounding autotrophic plants, significant 13C enrichments (ε13C) were found across all phenological stages, while significant 15N enrichment in the florescence period and strong 15N depletion during the fruiting period were found for both Bletilla species. We can deduce that both Bletilla species obtained carbon from root endophytic fungi during the whole year. Additionally, the temporal varying tendency of root endophytic fungal diversity was consistent with 13C enrichments, which was also accord with the nutritional requirement of plant.
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影响因子：
3
作者：
Andersson, S
通讯作者：
Andersson, S
DOI：
10.1098/rspb.2004.2807
发表时间：
2004-09-07
影响因子：
4.7
作者：
Bidartondo, MI;Burghardt, B;Read, DJ
通讯作者：
Read, DJ
影响因子：
--
作者：
Jin, Weitao;Xiang, Xiaoguo;Jin, Xiaohua
通讯作者：
Jin, Xiaohua
DOI：
10.1080/10256019308046133
发表时间：
1993-01-01
期刊：
ISOTOPENPRAXIS
影响因子：
--
作者：
GEBAUER, G;DIETRICH, P
通讯作者：
DIETRICH, P
影响因子：
3
作者：
Bailarote, Bruno Cachapa;Lievens, Bart;Jacquemyn, Hans
通讯作者：
Jacquemyn, Hans