Serum and sputum neurotrophin levels in chronic persistent cough
Serum and sputum neurotrophin levels in chronic persistent cough
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DOI：
10.1111/j.1365-2222.2005.02286.x
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发表时间：
2005-07-01
影响因子：
6.1
通讯作者：
Thomson, NC
中科院分区：
文献类型：
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作者：
Chaudhuri, R;McMahon, AD;Thomson, NC
背景神经营养因子（Neurotrophins，NTs）是一个生长因子家族，包括神经生长因子（nervegrowthfactor，NGF）、脑源性神经营养因子（brain-derivedneurotrophicfactor，BDNF）和神经营养因子3（neurotrophin 3，NT-3），它们参与炎症反应。血清和诱导痰NT水平增加，哮喘和咳嗽，因为特发性肺纤维化，分别。神经源性炎症与胸片正常的个体慢性咳嗽的发病机制有关，但NTs在这种情况下的作用是未知的。目的评估慢性持续性咳嗽患者血清和气道中NT水平是否升高。30例健康受试者为对照组。采用酶免疫法测定血清中神经生长因子（NGF）、脑源性神经营养因子（BDNF）和神经营养因子3（NT-3）的含量。在一个子集中，在诱导痰中测量NGF。痰液细胞计数和过敏原特异性血清IgE的测定和所有患者接受特定的序贯治疗试验，以达到最终诊断的couch.Results有没有显着差异，无论是在血清或痰NTs的水平在慢性咳嗽受试者与对照组相比，或咳嗽的最常见的原因：鼻后滴漏综合征，胃食管反流病，哮喘和支气管扩张症。健康对照组痰液NGF（pg/mL）的中位数（四分位数间距）为516（296-772），慢性咳嗽受试者为580（312-880）（P=0.284）。NT水平与痰细胞计数之间没有相关性。结论：慢性持续性咳嗽患者诱导痰和血清中神经营养素水平均未升高，且与咳嗽持续时间相关（r=0.34，P=0.002）。这意味着NTs在慢性持续性咳嗽的气道炎症中没有核心作用。
Background Neurotrophins (NTs) are a family of growth factors, including nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin3 (NT-3) that are involved in inflammation. Serum and induced sputum NT levels are increased in asthma and in cough because of idiopathic pulmonary fibrosis, respectively. Neurogenic inflammation is implicated in the pathogenesis of chronic cough in individuals with normal chest radiography, but the role of NTs in this condition is unknown.Objective To assess if NT levels are elevated in the serum and airways in subjects with chronic persistent cough.Methods Eighty-one subjects with chronic cough persistent for over 1 year; with normal chest radiography and spirometry were included. Thirty healthy subjects were controls. Serum NGF, BDNF and NT-3 were measured by enzyme immumoassay. In a subset, NGF was measured in induced sputum. Sputum cell counts and allergen-specific serum IgE were measured and all patients received specific sequential treatment trials to achieve a final diagnosis for the cough.Results There was no significant difference either in the levels of serum or sputum NTs in chronic cough subjects compared with controls or between the most common causes of cough: post-nasal drip syndrome, gastro-oesophageal reflux disease, asthma and bronchiectasis. The median (inter-quartile range) for sputum NGF (pg/mL) was 516 (296-772) in healthy controls and 580 (312-880) in subjects with chronic cough (P=0.284). There was no correlation between NT levels and sputum cell counts. Sputum NGF levels correlated with duration of cough (r=0.34, P=0.002).Conclusion: NTs are not elevated in induced sputum or serum of subjects with chronic persistent cough. This implies that NTs do not have a central role in perpetuating airway inflammation in chronic persistent cough.