Measuring Receptor Target Coverage: A Radioligand Competition Binding Protocol for Assessing the Association and Dissociation Rates of Unlabeled Compounds
Measuring Receptor Target Coverage: A Radioligand Competition Binding Protocol for Assessing the Association and Dissociation Rates of Unlabeled Compounds
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DOI：
10.1002/0471141755.ph0914s50
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发表时间：
2010-09
影响因子：
--
通讯作者：
D. Sykes;M. Dowling;S. Charlton
中科院分区：
文献类型：
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作者：
D. Sykes;M. Dowling;S. Charlton
配体-受体相互作用的动力学是新候选药物先导物优化的重要特征。本单元中描述的方案是一个动态放射性配体竞争结合试验，可以确定未标记受体配体的结合率和解离率。咕咕叫。Protoc。杂志,50:9.14.1 9.14.30应承担的。©2010 by John Wiley & Sons, Inc。
The kinetics of the ligand‐receptor interaction is an important feature in lead optimization for new drug candidates. The protocol described in this unit is a kinetic radioligand competition binding assay that makes possible the determination of both the association and dissociation rates of unlabeled receptor ligands. Curr. Protoc. Pharmacol. 50:9.14.1‐9.14.30. © 2010 by John Wiley & Sons, Inc.