Methods for studying store-operated calcium entry.
Methods for studying store-operated calcium entry.
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DOI：
10.1016/j.ymeth.2008.09.009
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发表时间：
2008-11
期刊：
影响因子：
4.8
通讯作者：
Putney, James W., Jr.
中科院分区：
文献类型：
--
作者：
Bird, Gary S.;DeHaven, Wayne I.;Smyth, Jeremy T.;Putney, James W., Jr.
关键词：
与磷脂酶C偶联的表面膜受体的活化导致细胞质Ca 2+信号的产生，包括细胞内Ca 2+释放和Ca 2+穿过质膜的增强进入。这种Ca 2+进入过程的主要机制归因于钙库操作的Ca 2+进入，这是一种通过1，4，5-三磷酸肌醇从细胞内钙库消耗Ca 2+离子而激活的过程。我们对Ca 2+释放和钙库操作的Ca 2+进入机制的理解已经从实验方法发展而来，这些方法包括使用荧光Ca 2+指示剂和电生理技术。药理学操纵的钙离子信号传导过程已受到一定的限制，但最近确定的关键分子球员，STIM和奥赖家族蛋白，提供了新的方法。在这里，我们描述了实用的方法，涉及荧光Ca 2+指示剂和电生理方法解剖观察到的细胞内Ca 2+信号，以揭示存储操作的Ca 2+进入的特性，突出这些方法的优点和局限性。
Activation of surface membrane receptors coupled to phospholipase C results in the generation of cytoplasmic Ca2+ signals comprised of both intracellular Ca2+ release, and enhanced entry of Ca2+ across the plasma membrane. A primary mechanism for this Ca2+ entry process is attributed to store-operated Ca2+ entry, a process that is activated by depletion of Ca2+ ions from an intracellular store by inositol 1,4,5-trisphosphate. Our understanding of the mechanisms underlying both Ca2+ release and store-operated Ca2+ entry have evolved from experimental approaches that include the use of fluorescent Ca2+ indicators and electrophysiological techniques. Pharmacological manipulation of this Ca2+ signaling process has been somewhat limited; but recent identification of key molecular players, STIM and Orai family proteins, has provided new approaches. Here we describe practical methods involving fluorescent Ca2+ indicators and electrophysiological approaches for dissecting the observed intracellular Ca2+ signal to reveal characteristics of store-operated Ca2+ entry, highlighting the advantages, and limitations, of these approaches.
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影响因子：
3.6
作者：
Braun, FJ;Aziz, O;Putney, JW
通讯作者：
Putney, JW
影响因子：
4.8
作者：
Broad, LM;Braun, FJ;Putney, JW
通讯作者：
Putney, JW
影响因子：
2.4
作者：
Fierro, L;Parekh, AB
通讯作者：
Parekh, AB
影响因子：
4
作者：
Bakowski, D;Parekh, AB
通讯作者：
Parekh, AB
影响因子：
4.8
作者：
DeHaven, Wayne I.;Smyth, Jeremy T.;Putney, James W., Jr.
通讯作者：
Putney, James W., Jr.