Novel and Efficient Synthesis of Phenethyl Formate via Enzymatic Esterification of Formic Acid
Novel and Efficient Synthesis of Phenethyl Formate via Enzymatic Esterification of Formic Acid
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DOI：
10.3390/biom10010070
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发表时间：
2020-01-01
期刊：
影响因子：
5.5
通讯作者：
Park, Chulhwan
中科院分区：
文献类型：
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作者：
Shin, Minguk;Seo, Jeongbae;Park, Chulhwan
目前生产酯的方法，包括化学合成和从天然来源中提取，由于产率低和环境污染而受到阻碍。这些化合物的酶促合成可以帮助克服这些问题。本研究以商用固定化脂肪酶为原料，合成了具有商业价值的甲酸苯乙酯。确定了最佳合成条件：Novozym435酶用量为15g/L，甲酸与苯乙醇的摩尔比为1：5，反应温度为40℃，溶剂为1，2-二氯乙烷。在此条件下，甲酸苯乙酯的转化率为95.92%。此外，以甲苯为溶剂取代1，2-二氯乙烷，酶可循环使用至少20次，转化率稳定在92%以上，证明了该工艺的经济性。与目前在学术和实验室中使用的方法相比，使用该方法的酶法合成甲酸苯乙酯更环保。此外，由于甲酸在商业必需酯生产中的下游使用，该方法有可能提高甲酸的附加值。
Current methods for the production of esters, including chemical synthesis and extraction from natural sources, are hindered by low yields and environmental pollution. The enzymatic synthesis of these compounds could help overcome these problems. In this study, phenethyl formate, a commercially valuable formate ester, was synthesized using commercial immobilized lipases. The effects of specific enzymes, enzyme concentration, formic acid:phenethyl alcohol molar ratio, temperature, and solvent were studied in order to optimize the synthesis conditions, which were identified as 15 g/L of Novozym 435 enzyme, a 1:5 formic acid:phenethyl alcohol molar ratio, a 40 degrees C reaction temperature, and 1,2-dichloroethane as the solvent. Under these conditions, phenethyl formate was obtained in a conversion yield of 95.92%. In addition, when 1,2-dichloroethane was replaced with toluene as the solvent, the enzyme could be recycled for at least 20 reactions with a steady conversion yield above 92%, testifying to the economic aspects of the process. The enzymatic synthesis of phenethyl formate using the proposed method is more environmentally friendly than methods currently employed in academic and laboratory settings. Moreover, the method has the potential to enhance the value-added properties of formic acid owing to its downstream use in the production of commercially essential esters.