Insights into the venom composition and evolution of an endoparasitoid wasp by combining proteomic and transcriptomic analyses.
Insights into the venom composition and evolution of an endoparasitoid wasp by combining proteomic and transcriptomic analyses.
复制标题
通过结合蛋白质组学和转录组学分析，深入了解内寄生蜂的毒液成分和进化
DOI：
10.1038/srep19604
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发表时间：
2016-01-25
影响因子：
4.6
通讯作者：
Ye G
中科院分区：
文献类型：
--
作者：
Yan Z;Fang Q;Wang L;Liu J;Zhu Y;Wang F;Li F;Werren JH;Ye G
寄生蜂是一种种类繁多的膜翅目昆虫，它们将卵产入寄主的体内(内寄生蜂)或外表面(外寄生蜂)。为了给黄蜂幼虫创造更有利的环境，外寄生蜂和内寄生蜂都向宿主注射毒液，以调节宿主的免疫、新陈代谢和发育。在膜翅目不同的谱系中，内寄生蜂都是由外寄生蜂独立进化而来的。翅目幼虫是各种蝴蝶的一种蛹内寄生蜂，代表了翅目内内寄生的一种相对较新的进化。利用转录组学和蛋白质组学相结合的方法，我们已经鉴定了70个可能存在于蚕蛾体内的毒素蛋白。它们中的大多数与近缘关系较远的内寄生蜂的毒液蛋白具有较高的相似性。此外，13种毒液蛋白与远亲内寄生蜂的毒液相似，但在Nasonia中没有检测到的毒液匹配。这些毒液蛋白可能在适应内寄生方面起作用。总体而言，这些结果为更详细地研究毒液功能和适应内寄生生活方式奠定了基础。
Parasitoid wasps are abundant and diverse hymenopteran insects that lay their eggs into the internal body (endoparasitoid) or on the external surface (ectoparasitoid) of their hosts. To make a more conducive environment for the wasps’ young, both ecto- and endoparasitoids inject venoms into the host to modulate host immunity, metabolism and development. Endoparasitoids have evolved from ectoparasitoids independently in different hymenopteran lineages. Pteromalus puparum, a pupal endoparasitoid of various butterflies, represents a relatively recent evolution of endoparasitism within pteromalids. Using a combination of transcriptomic and proteomic approaches, we have identified 70 putative venom proteins in P. puparum. Most of them show higher similarity to venom proteins from the related ectoparasitoid Nasonia vitripennis than from other more distantly related endoparasitoids. In addition, 13 venom proteins are similar to venoms of distantly related endoparasitoids but have no detectable venom matches in Nasonia. These venom proteins may have a role in adaptation to endoparasitism. Overall, these results lay the groundwork for more detailed studies of venom function and adaptation to the endoparasitic lifestyle.