Silencing Id-1 inhibits lymphangiogenesis through down-regulation of VEGF-C in oral squamous cell carcinoma
Silencing Id-1 inhibits lymphangiogenesis through down-regulation of VEGF-C in oral squamous cell carcinoma
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DOI：
10.1016/j.oraloncology.2010.10.012
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发表时间：
2011-01-01
期刊：
影响因子：
4.8
通讯作者：
Liu, Shaohua
中科院分区：
文献类型：
--
作者：
Dong, Zuoqing;Wei, Fengcai;Liu, Shaohua
我们的前期研究表明分化抑制因子Id-1（inhibitor of differentiation/DNA binding）的过度表达与口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）的淋巴结转移有关。本研究进一步揭示了Id-1与血管内皮生长因子-C（VEGF-C）和肿瘤周围淋巴管密度（PLVD）的关系，以及沉默Id-1对抑制口腔鳞癌淋巴管生成的作用。我们发现Id-1与VEGF-C（r = 0.569，p < 0.001）和PLVD（r = 0.240，p < 0.001）相关。慢病毒介导的RNA干扰在口腔鳞癌细胞系Tca 8113中靶向Id-1，导致VEGF-C下调（p = 0.003，0.007）。此外，当通过将Id-1-siRNA-慢病毒注射到裸鼠的移植肿瘤中来抑制Id-1时，VEGF-C下调（p = 0.018）并且PLVD降低（p = 0.001）。我们的研究结果表明，Id-1与淋巴管生成在口腔鳞癌。沉默Id-1可通过下调VEGF-C表达抑制淋巴管生成，有望成为治疗口腔鳞癌淋巴道转移的有效方法。(C)2010爱思唯尔有限公司版权所有。
Our previous study demonstrated that overexpression of Id-1 (inhibitor of differentiation/DNA binding) was associated with lymphatic metastasis in human oral squamous cell carcinoma (OSCC). In this study, we further unveiled the association of Id-1 with vascular endothelial growth factor-C (VEGF-C) and peritumoral lymphatic vessel density (PLVD), and the effect of silencing Id-1 on inhibiting lymphangiogenesis in OSCC. We found that Id-1 was associated with VEGF-C (r = 0.569, p < 0.001) and PLVD (r = 0.240, p < 0.001) in OSCC. Lentivirus-mediated RNA interference targeting Id-1 in an OSCC cell line Tca8113 resulted in down-regulation of VEGF-C (p = 0.003, 0.007). Moreover, when Id-1 was suppressed by injecting Id-1-siRNA-lentivirus into the transplanted tumors in nude mice, VEGF-C was down-regulated (p = 0.018) and the PLVD decreased (p = 0.001). Our results suggest that Id-1 was correlated with lymphangiogenesis in OSCC. Silencing Id-1 could inhibit lymphangiogenesis through down-regulation of VEGF-C and it might be a promising treatment modality for the lymphatic metastasis of OSCC. (C) 2010 Elsevier Ltd. All rights reserved.