The receptor potential of frog taste cells in response to cold and warm stimuli.
The receptor potential of frog taste cells in response to cold and warm stimuli.
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DOI：
10.1093/chemse/bjq039
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发表时间：
2010-07
期刊：
影响因子：
3.5
通讯作者：
Toshihide Sato;K. Nishishita;Y. Okada;K. Toda
中科院分区：
文献类型：
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作者：
Toshihide Sato;K. Nishishita;Y. Okada;K. Toda
研究了蛙味觉细胞的温度敏感性。命名为I型热敏（TS）细胞的味细胞在30 ℃的热刺激下去极化，在10 ℃的冷刺激下超极化。命名为TS II型的味觉细胞被冷刺激去极化，被热刺激超极化。在20 ℃下，选择性激活对冷刺激敏感的瞬时受体电位（TRP）M8通道的Menatrium溶液使TS II型细胞去极化，但不使TS I型细胞去极化。热刺激诱导的受体电位均被非选择性阳离子通道阻断剂氟灭酸阻断。结果表明，TS Ⅰ型细胞只有热感受通道，TS Ⅱ型细胞只有冷感受通道，两种通道均为非选择性阳离子通道。从公布的数据来看，TS II型细胞中冷传感器通道的候选者是TRPM 8通道，而TS I型细胞中热传感器通道的候选者可能是TRPM 4类通道。在味觉细胞的一个子集中，发现了TS III型和TS IV型细胞，前者在冷刺激和热刺激下均发生去极化，而后者在冷刺激和热刺激下均发生超极化。TS III和TS IV型细胞可能同时具有TRPM4样和TRPM8通道。在TS Ⅲ型细胞中，冷刺激和热刺激引起的去极化均占优势，而在TS Ⅳ型细胞中，热刺激引起的超极化均占优势。
Temperature sensitivity of frog taste cells was studied. The taste cell designated Type thermosensitive (TS) I cell was depolarized by warm stimulus at 30 degrees C and hyperpolarized by cold stimulus at 10 degrees C. The taste cell designated Type TS II cell was depolarized by the cold stimulus and hyperpolarized by the warm stimulus. Menthol solution at 20 degrees C, which selectively activates transient receptor potential (TRP) M8 channels sensitive to cold stimuli, depolarized Type TS II cells but not Types TS I cells. Thermal stimuli-induced receptor potentials were all blocked by a nonselective cation channel blocker flufenamic acid. The results indicate that Type TS I cells have warm sensor channels alone, Type TS II cells have cold sensor channels alone and both the channels are a nonselective cation channel. The candidate of cold sensor channel in Type TS II cells is a TRPM8 channel and that of warm sensor channel in Type TS I cells is likely to be a TRPM4-like channel from the published data. In a subset of taste cells, Types TS III and TS IV cells were found. The former was depolarized by both cold and warm stimuli, but the latter was hyperpolarized by both stimuli. Types TS III and TS IV cells might have both TRPM4-like and TRPM8 channels. It is supposed that depolarizations induced by both cold and warm stimuli were dominant in Type TS III cells and hyperpolarizations induced by both the thermal stimuli were dominant in Type TS IV cells.