Nitric oxide stimulates progesterone and prostaglandin E2 secretion as well as angiogenic activity in the equine corpus luteum
Nitric oxide stimulates progesterone and prostaglandin E2 secretion as well as angiogenic activity in the equine corpus luteum
复制标题
DOI：
10.1016/j.domaniend.2010.08.001
复制
发表时间：
2011-01-01
影响因子：
2.1
通讯作者：
Skarzynski, D. J.
中科院分区：
文献类型：
--
作者：
Ferreira-Dias, G.;Costa, A. S.;Skarzynski, D. J.
细胞因子和一氧化氮（NO）是黄体发育和维持、血管生成和血流的潜在介质。本研究的目的是评估（i）内皮和诱导型一氧化氮合酶的定位和蛋白表达（ii）一氧化氮供体的作用（精胺NONOate，NONOate）对孕酮（P4）和前列腺素（PG）E-2和因子（S）产生的影响刺激血管内皮细胞增殖。将黄体组织分类为黄体（CH; n = 5）、黄体中期CL（中期CL; n = 5）或黄体晚期CL（晚期CL; n = 5）。在整个黄体期，eNOS和iNOS均定位于大的黄体细胞和内皮细胞。eNOS表达在中CL期最低（P < 0.05），晚期最高（P < 0.05）。而iNOS表达无明显变化。在不添加激素或添加NONOate（10（-5）M）、肿瘤坏死因子-α（TNF α; 10 ng/mL;阳性对照）或马LH（100 ng/mL;阳性对照）的情况下培养黄体外植体。检测黄体组织条件培养基中P4和PGE（2）的含量及其刺激牛主动脉内皮细胞（BAEC）增殖的能力。所有治疗刺激释放的P4在CH，但不是在中间CL。TNF-α和NONOate治疗也增加了CH中PGE（2）水平和BAEC增殖（P < 0.05）。然而，在CL中期，无论使用何种治疗，均未观察到变化。这些数据表明NO和TNF α刺激马CH分泌功能和血管生成因子的产生。此外，在黄体发育早期，当血管发育更强烈时，NO可能在CL生长中发挥作用。(C)2011 Elsevier Inc. All rights reserved.
Cytokines and nitric oxide (NO) are potential mediators of luteal development and maintenance, angiogenesis, and blood flow. The aim of this study was to evaluate (i) the localization and protein expression of endothelial and inducible nitric oxide synthases (eNOS and iNOS) in equine corpora lutea (CL) throughout the luteal phase and (ii) the effect of a nitric oxide donor (spermine NONOate, NONOate) on the production of progesterone (P4) and prostaglandin (PG) E-2 and factor(s) that stimulate endothelial cell proliferation using equine luteal explants. Luteal tissue was classified as corpora hemorrhagica (CH; n = 5), midluteal phase CL (mid-CL; n = 5) or late luteal phase CL (late CL; it = 5). Both eNOS and iNOS were localized in large luteal cells and endothelial cells throughout the luteal phase. The expression of eNOS was the lowest in mid-CL (P < 0.05) and the highest in late CL (P < 0.05). However, no change was found for iNOS expression. Luteal explants were cultured with no hormone added or with NONOate (10(-5) M), tumor necrosis factor-alpha (TNF alpha; 10 ng/mL; positive control), or equine LH (100 ng/mL; positive control). Conditioned media by luteal tissues were assayed for P4 and PGE(2) and for their ability to stimulate proliferation of bovine aortic endothelial cells (BAEC). All treatments stimulated release of P4 in CH, but not in mid-CL. TNF alpha and NONOate treatments also increased PGE(2) levels and BAEC proliferation in CH (P < 0.05). However, in mid-CL, no changes were observed, regardless of the treatments used. These data suggest that NO and TNF alpha stimulate equine CH secretory functions and the production of angiogenic factor(s). Furthermore, in mares, NO may play a role in CL growth during early luteal development, when vascular development is more intense. (C) 2011 Elsevier Inc. All rights reserved.