Production of hypotaurine from l-cysteinesulfinate by rat liver mitochondria
Production of hypotaurine from l-cysteinesulfinate by rat liver mitochondria
复制标题
大鼠肝线粒体从L-半胱氨酸亚磺酸生产亚牛磺酸
DOI：
10.1007/s00726-007-0633-x
复制
发表时间：
2008
期刊：
影响因子：
3.5
通讯作者：
H. Nakamura
中科院分区：
文献类型：
--
作者：
T. Ubuka;A. Okada;H. Nakamura
摘要。亚牛磺酸是由L - 半胱亚磺酸生成牛磺酸的前体。人们认为肝脏中的亚牛磺酸生成发生在细胞质中。在本研究中，对大鼠肝脏线粒体中由L - 半胱亚磺酸生成亚牛磺酸的过程进行了研究。按照霍格布姆（Hogeboom）的方法制备线粒体，并使用0.25 M蔗糖溶液反复洗涤，然后将其与L - 半胱亚磺酸一起孵育。产物用丹磺酰氯进行衍生化，丹磺酰化的氨基酸通过反相高效液相色谱（RP - HPLC）进行分析。证实存在一个与丹磺酰 - 亚牛磺酸相对应的峰。通过过氧化氢处理，丹磺酰 - 亚牛磺酸的峰定量地转化为丹磺酰 - 牛磺酸。反应的最适pH在6.0到7.8之间较宽，L - 半胱亚磺酸的米氏常数（Km）为0.11 mM。结果表明肝脏线粒体中存在L - 半胱亚磺酸脱羧酶活性。总结了线粒体中的半胱氨酸代谢，并讨论了包括亚牛磺酸在内的半胱氨酸代谢产物在线粒体中可能具有的抗氧化作用。
Summary.Hypotaurine is the precursor of taurine production from l-cysteinesulfinate. It is recognized that hypotaurine production in the liver occurs in cytosol. In the present study, hypotaurine production from l-cysteinesulfinate in rat liver mitochondria was investigated. The mitochondrial preparation prepared according to the method of Hogeboom and washed repeatedly with 0.25 M sucrose solution was incubated with l-cysteinesulfinate. Products were derivatized with dabsyl chloride and dabsylated amino acids were analyzed by RP-HPLC. Presence of a peak corresponding to dabsyl-hypotaurine was confirmed. The peak of dabsyl-hypotaurine was converted quantitatively to dabsyl-taurine by the treatment with H2O2. Optimum pH of the reaction was shown to be broad between 6.0 and 7.8 and Km for l-cysteinesulfinate was 0.11 mM. Results indicate the presence of l-cysteinesulfinate decarboxylase activity in liver mitochondria. Mitochondrial cysteine metabolism was summarized and possible antioxidant roles of cysteine metabolites including hypotaurine in mitochondria are discussed.
DOI：
10.1016/s0021-9258(18)33025-4
发表时间：
1983-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
O. Griffith
通讯作者：
O. Griffith
多种形式的大鼠肝脏半胱氨酸亚磺酸脱羧酶。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Weinstein,CL;Griffith,OW
通讯作者：
Griffith,OW