First instance of settlement by cryopreserved coral larvae in symbiotic association with dinoflagellates
First instance of settlement by cryopreserved coral larvae in symbiotic association with dinoflagellates
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DOI：
10.1038/s41598-019-55374-6
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发表时间：
2019-12-11
影响因子：
4.6
通讯作者：
Lin, Chiahsin
中科院分区：
文献类型：
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作者：
Cirino, Luca;Wen, Zhi-Hong;Lin, Chiahsin
由于人类的影响，珊瑚礁在全球范围内受到影响，因此有必要进行低温保存。本研究的目的是开发一个合适的玻璃化和激光加热协议的石珊瑚Seriatopora caliendrum，垂直继承他们的甲藻共生体的幼虫。用冷冻保护剂（CPA）乙二醇（EG）、丙二醇（PG）、二甲亚砜（DMSO）、甘油（GLY）和甲醇（METH;按毒性从低到高的顺序列出）进行毒性实验，并使用2 M EG + 1 M PG和2 M EG + 1 M DMSO对幼虫进行玻璃化冷冻和激光加温。玻璃化和激光加热（300 V，10 ms脉冲宽度，2 mm光束直径），以1.2 x 10（18）GNB/mL的终浓度和535 nm的特征波长，使用2 MEG + 1 M PG、40%w/v Ficoll和10%v/v金纳米棒（GNB）的玻璃化溶液，产生具有55%和9%的活力和沉降百分比的幼虫，分别这是第一次成功地冷冻保存珊瑚幼虫，这些珊瑚幼虫在变暖时继续定居，并表明玻璃化和超快激光加热方法可能适用于其他受威胁的海洋物种。
Coral reefs are suffering on a global scale due to human impacts, thereby necessitating cryopreservation efforts. The objective of this study was to develop a suitable vitrification and laser warming protocol for larvae of the scleractinian coral Seriatopora caliendrum, which inherit their dinoflagellate algal symbionts vertically. Toxicity experiments were conducted with the cryoprotectants (CPAs) ethylene glycol (EG), propylene glycol (PG), dimethyl sulfoxide (DMSO), glycerol (GLY), and methanol (METH; listed in order from least to most toxic), and larvae were subjected to vitrification and laser warming using 2 M EG + 1 M PG and 2 M EG + 1 M DMSO. Vitrification and laser warming (300V, 10 ms pulse width, 2 mm beam diameter) using a vitrification solution of 2 M EG + 1 M PG, 40% w/v Ficoll, and 10% v/v gold nanobars (GNB) at a final concentration of 1.2 x 10(18) GNB/mL and a characteristic wavelength of 535 nm resulted in larvae with vitality and settlement percentages of 55 and 9%, respectively. This represents the first successful instance of cryopreservation of coral larvae that proceeded to settle upon warming, and suggests that the vitrification and ultra-fast laser warming approach may be applicable to other threatened marine species.