Resveratrol treatment rescues hyperleptinemia and improves hypothalamic leptin signaling programmed by maternal high-fat diet in rats
Resveratrol treatment rescues hyperleptinemia and improves hypothalamic leptin signaling programmed by maternal high-fat diet in rats
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DOI：
10.1007/s00394-015-0880-7
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发表时间：
2016-03-01
影响因子：
5
通讯作者：
Trevenzoli, I. H.
中科院分区：
文献类型：
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作者：
Franco, J. G.;Dias-Rocha, C. P.;Trevenzoli, I. H.
围产期高脂肪饮食与成年后代的肥胖和代谢疾病有关。白藜芦醇已被证明具有抗氧化和抗肥胖作用。然而，白藜芦醇对瘦素血症和瘦素信号传导的影响以及白藜芦醇治疗是否可以改善母亲高脂肪饮食规划的代谢结果仍不清楚。我们假设通过高脂肪饮食对雄性大鼠进行白藜芦醇治疗会降低体重和食物摄入量，并随着中枢瘦素信号的变化而降低瘦素血症。将雌性 Wistar 大鼠分为两组：对照组 (C)，接受含有 9% 脂肪热量的标准饮食；高脂组 (HF)，接受含有 28% 脂肪热量的饮食。交配前 8 周以及整个妊娠期和哺乳期，母鼠以 C 或 HF 日粮喂养。 C和HF雄性后代终生接受标准饮食。从 150 到 180 天龄，后代接受白藜芦醇（30 毫克/公斤体重/天）或载体（羧甲基纤维素）。与对照组相比，HF 后代体重增加、食欲亢进、皮下和内脏脂肪量增加，白藜芦醇治疗减少了肥胖。 HF 后代的瘦素血症增加，下丘脑弓状核中的 SOCS3 增加，这表明中枢瘦素抵抗。白藜芦醇治疗可挽救瘦素血症并增加下丘脑中的 p-STAT3 含量，而 SOCS3 没有变化，这表明瘦素信号传导有所改善。总的来说，我们的数据表明，白藜芦醇可以逆转高瘦素血症并改善 HF 母亲成年后代的中枢瘦素作用，从而减轻肥胖。
Perinatal high-fat diet is associated with obesity and metabolic diseases in adult offspring. Resveratrol has been shown to exert antioxidant and anti-obesity actions. However, the effects of resveratrol on leptinemia and leptin signaling are still unknown as well as whether resveratrol treatment can improve metabolic outcomes programmed by maternal high-fat diet. We hypothesize that resveratrol treatment in male rats programmed by high-fat diet would decrease body weight and food intake, and leptinemia with changes in central leptin signaling.Female Wistar rats were divided into two groups: control group (C), which received a standard diet containing 9 % of the calories as fat, and high-fat group (HF), which received a diet containing 28 % of the calories as fat. Dams were fed in C or HF diet during 8 weeks before mating and throughout gestation and lactation. C and HF male offspring received standard diet throughout life. From 150 until 180 days of age, offspring received resveratrol (30 mg/Kg body weight/day) or vehicle (carboxymethylcellulose).HF offspring had increased body weight, hyperphagia and increased subcutaneous and visceral fat mass compared to controls, and resveratrol treatment decreased adiposity. HF offspring had increased leptinemia as well as increased SOCS3 in the arcuate nucleus of the hypothalamus, which suggest central leptin resistance. Resveratrol treatment rescued leptinemia and increased p-STAT3 content in the hypothalamus with no changes in SOCS3, suggesting improvement in leptin signaling.Collectively, our data suggest that resveratrol could reverse hyperleptinemia and improve central leptin action in adult offspring from HF mothers attenuating obesity.