O-ring-induced transverse aortic constriction (OTAC) is a new simple method to develop cardiac hypertrophy and heart failure in mice.
O-ring-induced transverse aortic constriction (OTAC) is a new simple method to develop cardiac hypertrophy and heart failure in mice.
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DOI：
10.1038/s41598-021-04096-9
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发表时间：
2022-01-07
影响因子：
4.6
通讯作者：
Yamaguchi O
中科院分区：
文献类型：
--
作者：
Nakao Y;Aono J;Hamaguchi M;Takahashi K;Sakaue T;Inoue K;Ikeda S;Yamaguchi O
小鼠主动脉缩窄（TAC）是研究心脏压力超负荷引起的心力衰竭最常用的实验模型之一。然而，在传统的TAC中心力衰竭的发生率取决于操作者的技能。为了优化和简化这种方法，我们提出了O形环诱导的小鼠横向主动脉缩窄（OTAC）。对C57 BL/6 J小鼠进行OTAC，其中将O形环施加到横主动脉（在头臂动脉和左颈总动脉之间）并用三重结打结。我们使用不同内径的O型圈，分别为0.50和0.45 mm。OTAC的压力超负荷促进左心室（LV）肥大。OTAC还增加了肺重量，表明严重的肺充血。超声心动图结果显示，两个OTAC组均在术后一周内发生LV肥大，并逐渐降低LV缩短分数。此外，在OTAC小鼠的LV中观察到与心力衰竭、LV肥大和LV纤维化相关的基因表达显著升高。我们证明了OTAC方法，这是一种简单有效的小鼠心脏压力超负荷方法。这种方法将有效地帮助我们了解心力衰竭（HF）的机制，降低左心室射血分数（HFrEF）和心脏肥大。
Suture-based transverse aortic constriction (TAC) in mice is one of the most frequently used experimental models for cardiac pressure overload-induced heart failure. However, the incidence of heart failure in the conventional TAC depends on the operator’s skill. To optimize and simplify this method, we proposed O-ring-induced transverse aortic constriction (OTAC) in mice. C57BL/6J mice were subjected to OTAC, in which an o-ring was applied to the transverse aorta (between the brachiocephalic artery and the left common carotid artery) and tied with a triple knot. We used different inner diameters of o-rings were 0.50 and 0.45 mm. Pressure overload by OTAC promoted left ventricular (LV) hypertrophy. OTAC also increased lung weight, indicating severe pulmonary congestion. Echocardiographic findings revealed that both OTAC groups developed LV hypertrophy within one week after the procedure and gradually reduced LV fractional shortening. In addition, significant elevations in gene expression related to heart failure, LV hypertrophy, and LV fibrosis were observed in the LV of OTAC mice. We demonstrated the OTAC method, which is a simple and effective cardiac pressure overload method in mice. This method will efficiently help us understand heart failure (HF) mechanisms with reduced LV ejection fraction (HFrEF) and cardiac hypertrophy.
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