Expression of interleukin 6 in brain and colon of rats with TNBS-induced colitis.
Expression of interleukin 6 in brain and colon of rats with TNBS-induced colitis.
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DOI：
10.3748/wjg.v16.i18.2252
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发表时间：
2010-05
影响因子：
4.3
通讯作者：
Kai Wang;Chuan-Ping Yuan;Wei Wang;Zhanqing Yang;Wei Cui;Liangliang Mu;Z. Yue;Xiu-ling Yin;Zhong-Ming Hu;Juxiong Liu
中科院分区：
文献类型：
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作者：
Kai Wang;Chuan-Ping Yuan;Wei Wang;Zhanqing Yang;Wei Cui;Liangliang Mu;Z. Yue;Xiu-ling Yin;Zhong-Ming Hu;Juxiong Liu
目的 表征强效促炎细胞因子白细胞介素6（IL-6）在炎症性肠病（IBD）发生和发展中的表达，并探讨其对神经免疫调节和免疫稳态调节的影响。方法在本研究中，三硝基苯磺酸（TNBS）诱导的结肠炎大鼠在诱导后第3、7、14、21和28天处死。在对照中，TNBS仅被等量的磷酸盐缓冲溶液（PBS，0.01mol/L）替代。采用实时逆转录聚合酶链式反应评估各时相脑组织和结肠组织中IL-6 mRNA的表达量，并采用免疫组化法测定IL-6的细胞定位和蛋白水平。结果第7天时，IBD大鼠结肠和大脑中IL-6 mRNA的表达显着高于对照组。与对照组相比，IBD 大鼠的结肠、下丘脑和大脑皮层中的蛋白质水平也显着升高。因此，所有位置的 IL-6 mRNA 表达和蛋白质水平都存在类似的时间趋势，持续增加，在第 7 天达到峰值，然后下降并逐渐恢复到正常水平。结论这些结果揭示了IBD的不同阶段脑和结肠组织中IL-6表达的变化。因此，我们提出神经中枢通过IL-6调控IBD的发生和发展。
AIM To characterise expression of interleukin 6 (IL-6), a potent proinflammatory cytokine, in the occurrence and development of inflammatory bowel disease (IBD) and investigate its effect on neuroimmunomodulation and immune homeostasis regulation. METHODS In this study, rats with colitis induced by trinitrobenzene sulfonic acid (TNBS) were sacrificed on days 3, 7, 14, 21 and 28 after induction. In the controls, the TNBS was just replaced by equivalent amount of phosphate buffered solution (PBS, 0.01 mol/L). IL-6 mRNA expression in brain and colon tissues in each phase was evaluated by real-time reverse transcription-polymerase chain reaction, and cellular localisation and protein level of IL-6 was determined by immunohistochemistry. RESULTS At day 7, mRNA expression of IL-6 was significantly higher in the colon and brain of IBD rats than that of the controls. The protein level was also significantly higher in colon, hypothalamus and cerebral cortex of IBD rats compared with the controls. So there are similar temporal trends in IL-6 mRNA expression and protein levels in all positions with a persistent increase to a peak at day 7, followed by a decline and gradual return to normal levels. CONCLUSION These results revealed that changes in IL-6 expression in brain and colon tissues occur in different phases of IBD. Therefore, we propose that the nerve centre regulates and controls the occurrence and development of IBD via IL-6.