Quantum dot ex vivo labeling of neuromuscular synapses.
Quantum dot ex vivo labeling of neuromuscular synapses.
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DOI：
10.1021/nl072460x
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发表时间：
2008-02
期刊：
影响因子：
10.8
通讯作者：
Rebecca L. Orndorff;M. R. Warnement;J. N. Mason;R. Blakely;S. Rosenthal
中科院分区：
文献类型：
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作者：
Rebecca L. Orndorff;M. R. Warnement;J. N. Mason;R. Blakely;S. Rosenthal
烟碱受体（nAchRs）负责神经递质乙酰胆碱（Ach）的快速兴奋性信号传导。它们存在于神经肌肉连接（NMJs）的突触后膜上，也存在于脑突触上。α -bungarotoxin （α - btx）是一种高亲和力的nAchR拮抗剂，可抑制Ach结合和神经传递。在这里，我们证明生物素化的α - btx结合到天然小鼠膈nachr上，可以使用链霉亲和素共轭量子点在体外量化和可视化。这种方法为直接评估天然组织中神经递质受体的存在和移动性提供了一种新的方法。
Nicotinic receptors (nAchRs) are responsible for fast excitatory signaling by the neurotransmitter acetylcholine (Ach). They are present on the postsynaptic membrane at neuromuscular junctions (NMJs) and also at brain synapses. Alpha-bungarotoxin (alpha-BTX), a high-affinity nAchR antagonist, inhibits Ach binding and neurotransmission. Here we demonstrate biotinylated alpha-BTX, bound to native mouse diaphragm nAchRs, can be quantified and visualized ex vivo using streptavidin-conjugated quantum dots. This approach provides a novel methodology for the direct assessment of the presence and mobility of neurotransmitter receptors in native tissue.