The Hlx homeobox transcription factor is required early in enteric nervous system development
The Hlx homeobox transcription factor is required early in enteric nervous system development
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DOI：
10.1186/1471-213x-6-33
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发表时间：
2006-07-19
影响因子：
--
通讯作者：
Harvey, Richard P.
中科院分区：
文献类型：
--
作者：
Bates, Michael D.;Dunagan, Dana T.;Harvey, Richard P.
背景资料：肠神经系统（ENS）的发展需要迁移神经嵴细胞和新生胃肠道之间的相互作用，这取决于两个细胞室表达的基因。Hlx是一种在小鼠肠和肝间质中表达的同源异型盒转录因子基因，是肠和肝的正常胚胎生长所必需的，并且Hlx（-/-）基因型是胚胎致死的。我们假设Hlx是ENS development.Results所必需的：肠神经元被确定在Hlx（+/+）和Hlx（-/-）小鼠胚胎的神经标记PGP9.5和Phox 2b的胚胎切片的免疫染色，或通过染色β-半乳糖苷酶在含有多巴胺β-羟化酶-nLacZ转基因的整装胚胎。在Hlx（+/+）胚胎中，神经嵴细胞/肠神经元在E10.5时已从胃移动到肠中。相反，Hlx-/-胚胎的神经嵴细胞/肠神经元主要局限于胃侧间充质，E10.5- 16.5之间肠内仅有少量散在的神经嵴细胞/肠神经元。结论：Hlx同源框转录因子在ENS发育的早期阶段是必需的。
Background: Development of the enteric nervous system ( ENS) requires interactions between migrating neural crest cells and the nascent gastrointestinal tract that are dependent upon genes expressed by both cell compartments. Hlx, a homeobox transcription factor gene that is expressed in mouse intestinal and hepatic mesenchyme, is required for normal embryonic growth of intestine and liver, and the Hlx(-/-) genotype is embryonic lethal. We hypothesized that Hlx is required for ENS development.Results: Enteric neurons were identified in Hlx(+/+) and Hlx(-/-) mouse embryos by immunostaining of embryo sections for the neural markers PGP9.5 and Phox2b, or by staining for beta-galactosidase in whole-mount embryos containing the dopamine beta-hydroxylase-nLacZ transgene. In Hlx(+/+) embryos, neural crest cells/enteric neurons have moved from the stomach into the intestine by E10.5. By contrast, neural crest cells/enteric neurons remain largely restricted to the lateral stomach mesenchyme of Hlx-/- embryos, with only a few scattered neural crest cells/enteric neurons in the intestine between E10.5-16.5.Conclusion: The Hlx homeobox transcription factor is required for early aspects of ENS development.