Morphological characteristics of male germ cells of rats in contact with Sertoli cells in vitro.
Morphological characteristics of male germ cells of rats in contact with Sertoli cells in vitro.
复制标题
体外与支持细胞接触的大鼠雄性生殖细胞的形态特征。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
M. Stefanini
中科院分区：
文献类型：
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作者：
F. Palombi;E. Ziparo;F. Rommerts;J. Grootegoed;M. Antonini;M. Stefanini
取3周龄大鼠生精上皮片。虽然支持细胞形成单层，但生殖细胞(精原细胞和早期精母细胞)仍与它们联系在一起，具有正常的超微结构。在化学定义的培养液中培养3周后，生殖细胞与支持细胞完全分离。Sertoli与生殖细胞之间的接触区呈桥粒状连接，而生殖细胞与Sertoli之间的接触区呈五层状。
Fragments of seminiferous epithelium were prepared from 3-week-old rats. Although the Sertoli cells formed a monolayer, germ cells (spermatogonia and early spermatocytes) remained in association with them and were of normal ultrastructural appearance. Germ cells became completely separated from Sertoli cells after 3 weeks of culture in a chemically defined medium. The contact areas between Sertoli and germ cells were characterized by desmosome-like junctions while those between germ cells appeared to be pentalaminar.