Efficient persistence of extrachromosomal KSHV DNA mediated by latency-associated nuclear antigen
Efficient persistence of extrachromosomal KSHV DNA mediated by latency-associated nuclear antigen
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DOI：
10.1126/science.284.5414.641
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发表时间：
1999-04-23
期刊：
影响因子：
56.9
通讯作者：
Kaye, KM
中科院分区：
文献类型：
--
作者：
Ballestas, ME;Chatis, PA;Kaye, KM
原发积液淋巴瘤(PEL)细胞含有Kaposi肉瘤相关疱疹病毒(KSHV)表型，并表达KSHV编码的潜伏期相关核抗原(LANA)。在PEL细胞中，LANA和KSHV DNA共存于间期核和有丝分裂染色体上的圆点状。在没有KSHV DNA的情况下，LANA弥漫分布在细胞核或有丝分裂染色体上。在淋巴母细胞中，LANA是含有特定KSHV DNA片段的上体持续存在的必要条件和充分条件。此外，LANA与人工构建的KSHV DNA异构体共定位于细胞核和有丝分裂染色体上。这些结果支持一个模型，在该模型中，LANA在有丝分裂过程中将KSHV DNA拴在染色体上，从而使KSHV表观染色体能够有效地分离到后代细胞。
Primary effusion Lymphoma (PEL) cells harbor Kaposi's sarcoma-associated herpesvirus (KSHV) episomes and express a KSHV-encoded Latency-associated nuclear antigen (LANA). In PEL cells, LANA and KSHV DNA colocalized in dots in interphase nuclei and along mitotic chromosomes. In the absence of KSHV DNA, LANA was diffusely distributed in the nucleus or on mitotic chromosomes. In Lymphoblasts, LANA was necessary and sufficient for the persistence of episomes containing a specific KSHV DNA fragment. Furthermore, LANA colocalized with the artificial KSHV DNA episomes in nuclei and along mitotic chromosomes. These results support a model in which LANA tethers KSHV DNA to chromosomes during mitosis to enable the efficient segregation of KSHV episomes to progeny cells.