Drosophila Mcm10 interacts with members of the prereplication complex and is required for proper chromosome condensation
Drosophila Mcm10 interacts with members of the prereplication complex and is required for proper chromosome condensation
复制标题
DOI：
10.1091/mbc.e02-11-0706
复制
发表时间：
2003-06-01
影响因子：
3.3
通讯作者：
Tye, BK
中科院分区：
文献类型：
--
作者：
Christensen, TW;Tye, BK
Mcm10是酿酒酵母DNA复制起始所必需的。我们从果蝇中克隆了MCM10，并证明它与ScMCM10零突变体互补。此外，Mcm10与复制前复合体的关键成员Mcm2、Dup （Cdt1）和Orc2相互作用。Mcm10与自身、Cdc45和Hp1之间也检测到相互作用。KC细胞中Orc2和Mcm10的RNAi缺失导致DNA含量的损失。此外，Mcm10、Cdc45、Mcm2、Mcm5和Orc2的缺失分别导致染色体异常凝聚。观察到的冷凝缺陷类似于先前发表的Orc2、Orc5和Mcm4突变体的报告。我们的结果加强和扩展的论点，染色质凝聚和DNA复制的过程是联系在一起的。
Mcm10 is required for the initiation of DNA replication in Saccharomyces cerevisiae. We have cloned MCM10 from Drosophila melanogaster and show that it complements a ScMCM10 null mutant. Moreover, Mcm10 interacts with key members of the prereplication complex: Mcm2, Dup (Cdt1), and Orc2. Interactions were also detected between Mcm10 and itself, Cdc45, and Hp1. RNAi depletion of Orc2 and Mcm10 in KC cells results in loss of DNA content. Furthermore, depletion of Mcm10, Cdc45, Mcm2, Mcm5, and Orc2, respectively, results in aberrant chromosome condensation. The condensation defects observed resemble previously published reports for Orc2, Orc5, and Mcm4 mutants. Our results strengthen and extend the argument that the processes of chromatin condensation and DNA replication are linked.