T-lymphocyte subsets in smoking and lung cancer: Analysis of monoclonal antibodies and flow cytometry.
T-lymphocyte subsets in smoking and lung cancer: Analysis of monoclonal antibodies and flow cytometry.
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吸烟和肺癌中的 T 淋巴细胞亚群：单克隆抗体和流式细胞术分析。
DOI：
10.1164/arrd.1982.126.2.265
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Kazemi,H
中科院分区：
文献类型：
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作者：
Ginns,LC;Goldenheim,PD;Miller,LG;Burton,RC;Gillick,L;Colvin,RB;Goldstein,G;Kung,PC;Hurwitz,C;Kazemi,H
为了确定免疫调节性t细胞是否在肺癌患者中发生异常，我们对26例未经治疗的肺癌患者的外周血t淋巴细胞进行了表征。将原发性鳞状癌（SC） （n = 10）、原发性腺癌（AC） （n = 7）和继发性肺转移(M) （n = 9）患者的结果相互比较，并与非癌症患者（n = 48）进行比较，包括不吸烟者（n = 29）和吸烟者（n = 19）。我们发现，在轻度至中度吸烟者中，OKT3+（成熟的外周）t淋巴细胞，包括OKT4+（诱导剂/辅助剂）和OKT8+（细胞毒性/抑制因子）淋巴细胞增加，而在重度吸烟者中，OKT4+细胞减少（p < 0.05）。反映免疫调节细胞平衡的OKT4+ / OKT8+(4/8)淋巴细胞比值在轻、中度吸烟者中正常，在重度吸烟者中下降（p < 0.05）。SC患者的循环t细胞谱与吸烟者相似。相比之下，在AC患者中，我们发现OKT8+细胞的百分比下降（p < 0.05）。AC患者的4/8比值升高（p < 0.05）。在M患者中，OKT3+细胞的比例在OKT4+和OKT8+亚群中均有所下降。M患者的4/8比例较低。因此，在吸烟者和肺癌患者中都发现了循环t细胞的一些异常。这些结果提示免疫调节异常参与肺癌的发病机制。
In order to determine whether abnormalities of immunoregulatory T-cells occur in patients with lung cancer, we characterized peripheral T-lymphocytes in 26 patients with untreated lung cancer. The results in patients with primary squamous cancer (SC) (n = 10), primary adenocarcinoma (AC) (n = 7), and secondary lung metastases (M) (n = 9) were compared with each other and to subjects without cancer (n = 48), including nonsmokers (n = 29) and smokers (n = 19). We found that OKT3+(mature, peripheral) T-lymphocytes, including both OKT4+(inducer/helper) and OKT8+(cytotoxic/suppressor) lymphocytes, were increased in light-to-moderate smokers, but that OKT4+cells were decreased in heavy smokers (p < 0.05). The ratio of OKT4+to OKT8+(4/8) lymphocytes, reflecting the balance of immunoregulatory cells, was normal in light-to-moderate smokers, but was decreased in heavy smokers (p < 0.05). The profile of circulating T-cells in patients with SC was similar to the smokers. In contrast, in patients with AC, we found a decreased percentage of OKT8+cells (p < 0.05). The 4/8 ratio was elevated in patients with AC (p < 0.05). In patients with M, there was a decreased percentage of OKT3+cells reflected in both OKT4+and OKT8+subsets. The 4/8 ratio in patients with M was low. Thus, a number of abnormalities in circulating T-cells was found both in smokers and in patients with lung cancer. These results suggest that immunoregulatory abnormalities contribute to the pathogenesis of lung cancer.