Sinapic acid or its derivatives interfere with abscisic acid homeostasis during Arabidopsis thaliana seed germination.
Sinapic acid or its derivatives interfere with abscisic acid homeostasis during Arabidopsis thaliana seed germination.
复制标题
芥子酸或其衍生物干扰拟南芥种子萌发过程中脱落酸稳态
DOI：
10.1186/s12870-017-1048-9
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发表时间：
2017-06-06
影响因子：
5.3
通讯作者：
Miao Y
中科院分区：
文献类型：
--
作者：
Bi B;Tang J;Han S;Guo J;Miao Y
背景：辛酸及其酯在种子萌发和植物发育的不同阶段具有广泛的功能，并被认为具有抗紫外线照射的作用。为了更好地了解辛酸酯在不同胁迫下与种子萌发过程的相互作用，我们分析了植物激素脱落酸（ABA）在辛酸酯参与种子萌发和幼苗早期生长的调控中的作用。结果：我们发现外源辛酸促进拟南芥种子萌发呈剂量依赖性。高效液相色谱-质谱分析表明，外源sinapic酸增加了吸收种子中sinapoylcholine的含量。此外，辛酸影响ABA分解代谢，导致ABA水平降低和ABA-葡萄糖酯水平升高。利用缺乏sinap酸酯合成的突变体，我们发现突变体sinapoylglucose accumulator 2 （sng2）和bright trichomes 1 （brt1）种子的萌发比野生型对ABA更敏感。此外，缺乏脱落酸缺陷2 （ABA2）或脱落酸不敏感3 （ABI3）的拟南芥突变体在sinapic酸处理下显示出sinapoylglucose:choline sinapoyltransferase (SCT) and sinapoylcholine esterase （SCE）基因的表达增加。该处理还影响了种子萌发过程中sinapoylcholine和游离胆碱的积累。结论：在拟南芥种子萌发和幼苗早期生长过程中，作为主要酚类成分的sinapoylcholine影响了ABA的动态平衡。我们的研究结果揭示了辛酸及其酯在aba介导的拟南芥种子萌发抑制中对干旱胁迫的调控作用。
Background:Sinapic acid and its esters have broad functions in different stages of seed germination and plant development and are thought to play a role in protecting against ultraviolet irradiation. To better understand the interactions between sinapic acid esters and seed germination processes in response to various stresses, we analyzed the role of the plant hormone abscisic acid (ABA) in the regulation of sinapic acid esters involved in seed germination and early seedling growth.Results:We found that exogenous sinapic acid promotes seed germination in a dose-dependent manner in Arabidopsis thaliana. High-performance liquid chromatography mass spectrometry analysis showed that exogenous sinapic acid increased the sinapoylcholine content of imbibed seeds. Furthermore, sinapic acid affected ABA catabolism, resulting in reduced ABA levels and increased levels of the ABA-glucose ester. Using mutants deficient in the synthesis of sinapate esters, we showed that the germination of mutant sinapoylglucose accumulator 2 (sng2) and bright trichomes 1 (brt1) seeds was more sensitive to ABA than the wild-type. Moreover, Arabidopsis mutants deficient in either abscisic acid deficient 2 (ABA2) or abscisic acid insensitive 3 (ABI3) displayed increased expression of the sinapoylglucose:choline sinapoyltransferase (SCT) and sinapoylcholine esterase (SCE) genes with sinapic acid treatment. This treatment also affected the accumulation of sinapoylcholine and free choline during seed germination.Conclusions:We demonstrated that sinapoylcholine, which constitutes the major phenolic component in seeds among various minor sinapate esters, affected ABA homeostasis during seed germination and early seedling growth in Arabidopsis. Our findings provide insights into the role of sinapic acid and its esters in regulating ABA-mediated inhibition of Arabidopsis seed germination in response to drought stress.