Immunocytochemical colocalization of specific immunoglobulin A with sendai virus protein in infected polarized epithelium.
Immunocytochemical colocalization of specific immunoglobulin A with sendai virus protein in infected polarized epithelium.
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DOI：
10.1084/jem.188.7.1223
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发表时间：
1998-10-05
期刊：
影响因子：
--
通讯作者：
Mazanec MB
中科院分区：
文献类型：
--
作者：
Fujioka H;Emancipator SN;Aikawa M;Huang DS;Blatnik F;Karban T;DeFife K;Mazanec MB
Immunoglobulin (Ig)A provides the initial immune barrier to viruses at mucosal surfaces. Specific IgA interrupts viral replication in polarized epithelium during receptor-mediated transport, probably by binding to newly synthesized viral proteins. Here, we demonstrate by immunoelectron microscopy that specific IgA monoclonal antibodies (mAbs) accumulate within Sendai virus–infected polarized cell monolayers and colocalize with the hemagglutinin– neuraminidase (HN) viral protein in a novel intracellular structure. Neither IgG specific for HN nor irrelevant IgA mAbs colocalize with viral protein. Treatment of cultures with viral-specific IgA but not with viral-specific IgG or irrelevant IgA decreases viral titers. These observations provide definitive ultrastructural evidence of a subcellular compartment in which specific IgA and viral envelope proteins interact, further strengthening our hypothesis of intracellular neutralization of virus by specific IgA antibodies. Our results have important implications for intracellular protein trafficking, viral replication, and viral vaccine development.
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DOI：
10.1083/jcb.116.3.577
发表时间：
1992-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Mostov K;Apodaca G;Aroeti B;Okamoto C
通讯作者：
Okamoto C
DOI：
10.1006/smvy.1996.0034
发表时间：
1996-08-01
期刊：
SEMINARS IN VIROLOGY
影响因子：
--
作者：
Crowe, JE
通讯作者：
Crowe, JE
影响因子：
3.7
作者：
PORTNER, A;MURTI, KG
通讯作者：
MURTI, KG
MDCK细胞中IgA的受体介导的转介症是通过根尖回收的内体。
影响因子：
7.8
作者：
Apodaca, G;Katz, L A;Mostov, K E
通讯作者：
Mostov, K E
影响因子：
3.7
作者：
DESHPANDE, KL;PORTNER, A
通讯作者：
PORTNER, A