An approach to acoustic properties of biological tissues using acoustic micrographs of attenuation constant and sound speed
An approach to acoustic properties of biological tissues using acoustic micrographs of attenuation constant and sound speed
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DOI：
10.7863/jum.2001.20.8.891
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发表时间：
2001-08-01
影响因子：
2.3
通讯作者：
Nitta, S
中科院分区：
文献类型：
--
作者：
Okawai, H;Kobayashi, K;Nitta, S
目标。开发一种在微观水平上对厚度约为10微米的标本进行生物组织元素声学特性二维测量的方法。方法。该程序是在100至200 MHz频率范围内的机械扫描声显微技术和干涉现象理论的基础上开发的。制备了各种组织和样品来评估该方法并解释声音传播特性。结果。蛋白质含量高、水分含量低或两者兼而有之的组织具有较高的衰减常数和声速。在微观水平上，衰减随频率的指数n几乎是一致的，而当试样厚度较大时，衰减随频率的指数n大于1。未观察到声速分散。结论。该方法被证明是可重复的，并且根据组织类型和标本厚度对数据进行了声学和病理学解释。
Objective. To develop a method for two-dimensional measurement of acoustic properties of biological tissue elements at the microscopic level for specimens with a thickness of approximately 10 mum. Methods. The procedure was developed on the basis of mechanically scanned acoustic microscopic techniques in the frequency range of 100 to 200 MHz and the theory of interference phenomena. Various tissues and samples were prepared to evaluate the method and to interpret sound propagation properties. Results. Tissues with high protein content, low water content, or both had a high attenuation constant and sound speed. The exponent n of attenuation against frequency was almost unity at the microscopic level, whereas it was greater than unity when the specimen thickness was greater. Sound speed dispersion was not observed. Conclusions. The method was shown to be reproducible, and the data were interpreted acoustically and pathologically with reference to tissue type and specimen thickness.