Fine mapping ofqSdr9, a novel locus for seed dormancy (SD) in weedy rice, and development of NILs with a strong SD allele
Fine mapping ofqSdr9, a novel locus for seed dormancy (SD) in weedy rice, and development of NILs with a strong SD allele
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qSdr9（杂草稻种子休眠（SD）的新位点）的精细定位，以及具有强 SD 等位基因的 NIL 的开发
DOI：
10.1007/s11032-020-01148-2
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发表时间：
2020
影响因子：
3.1
通讯作者：
Wan Jianmin
中科院分区：
文献类型：
--
作者：
Nguyen Thanhliem;Fu Kai;Mou Changling;Yu Jiangfeng;Zhu Xingjie;Huang Yunshuai;Zhou Chunlei;Hao Qixian;Zhang Fulin;Song Weihan;Wang Ping;Chen Yaping;Ma Tengfei;Tian Yunlu;Liu Shijia;Jiang Ling;Wan Jianmin
种子缺乏休眠是水稻和其他谷类作物收获前发芽的主要原因，导致谷物产量和质量显著降低。杂草稻通常具有较强的休眠性，因此，杂草稻“陆稻”种子休眠性相关的数量性状位点（QTL）可用于改良粳稻品种“宁粳4号”的穗发芽抗性。为鉴定鲁稻SD QTL，以鲁稻为供体亲本，通过标记辅助回交，结合SD表型分析，构建了宁粳4号BC 6 F2群体。9 L染色体上的QTL qSdr 9解释了18.09%的表型变异，而鹭岛等位基因使发芽率降低了10.78%。将qSdr 9精细定位于标记QNU 9 -10和RM 5777之间的586.60 kb区域。利用与qSdr 9基因座紧密连锁的标记，以鲁稻为材料，以宁粳4号为背景，构建了qSdr 9基因近等基因系。具有强SD的qSdr 9近等基因系将在收获前发芽抗性育种中用作亲本。
Lack of seed dormancy, a major cause of preharvest sprouting in rice and other cereal crops, causes significant reductions in grain yield and quality. Weedy rice is often strongly dormant; therefore, quantitative trait loci (QTLs) related to seed dormancy in weedy rice “Ludao” could be useful for improving pre-harvest sprouting resistance in the important japonica variety “Ninggeng 4.” To identify SD QTLs in variety Ludao, we constructed an advanced backcross population (BC6F2) of Ninggeng 4 with Ludao as the donor parent by markerassisted backcrossing followed by SD phenotyping. The consistently detected QTL qSdr9 on chromosome 9L explained 18.09% of the phenotypic variance, and the Ludao allele decreased germination percentage by 10.78%. qSdr9 was fine-mapped to a 586.60 kb region between markers QNU9–10 and RM5777. Based on the closest linked marker for locus qSdr9, we developed near-isogenic lines with the qSdr9 allele from Ludao in Ninggeng 4 background. The qSdr9 near-isogenic lines with strong SD will be useful as parents in breeding for pre-harvest sprouting resistance.