Unbound free fatty acid profiles in human plasma and the unexpected absence of unbound palmitoleate
Unbound free fatty acid profiles in human plasma and the unexpected absence of unbound palmitoleate
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DOI：
10.1194/jlr.m074260
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发表时间：
2017-03-01
影响因子：
6.5
通讯作者：
Kleinfeld, Alan M.
中科院分区：
文献类型：
--
作者：
Huber, Andrew H.;Kleinfeld, Alan M.
我们首次确定了人血浆中9种最丰富的未结合FFA（FFAus）的分布。测定了脂质MAPSMAPS联盟已测定总FFA谱的合并健康成人标准参比血浆的谱。通过使用20种不同的丙烯酰胺标记的脂肪酸结合蛋白突变体（探针）进行测量，这些突变体对9种FFA具有互补特异性，这些FFA占长链血浆FFA的96%以上。每种探针的丙烯酰二氢呋喃荧光发射在结合FFAu时发生变化。通过组合20种探针的测量荧光比率来确定9种FFA中每一种的血浆浓度。总摩尔FFAu浓度占总FFA浓度的< 10（-5），并且FFAu分布的摩尔分数与总FFA分布显著不同。例如，肉豆蔻酸占未结合的22%，占总量的2.8%。最令人惊讶的差异是我们发现零未结合的顺式-9-棕榈油酸（POA），而总POA为7.2%。一种未鉴定的血浆成分似乎专门防止POA的释放。FFAu是具有生理活性的游离脂肪酸，血浆FFAu谱可以提供有关人类健康的新信息。
We determined for the first time the profiles of the nine most abundant unbound FFAs (FFAus) in human plasma. Profiles were determined for a standard reference plasma of pooled healthy adults for which the Lipid MAPSMAPS Consortium had determined the total FFA profiles. Measurements were performed by using 20 different acrylodan-labeled fatty acid binding protein mutants (probes), which have complementary specificities for the nine FFAs that comprise more than 96% of long-chain plasma FFA. The acrylodan fluorescence emission for each probe changes upon binding a FFAu. The plasma concentrations of each of the nine FFAus were determined by combining the measured fluorescence ratios of the 20 probes. The total molar FFAu concentration accounted for < 10(-5) of the total FFA concentration, and the mole fractions of the FFAu profiles were substantially different than the total FFA profiles. Myristic acid, for example, comprises 22% of the unbound versus 2.8% of the total. The most surprising difference is our finding of zero unbound cis-9-palmitoleic acid (POA), whereas the total POA was 7.2%. An unidentified plasma component appears to specifically prevent the release of POA. FFAus are the physiologically active FFAs, and plasma FFAu profiles may provide novel information about human health.