Food entrainment modifies the c-Fos expression pattern in brain stem nuclei of rats
Food entrainment modifies the c-Fos expression pattern in brain stem nuclei of rats
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DOI：
10.1152/ajpregu.00590.2004
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发表时间：
2005-03-01
影响因子：
2.8
通讯作者：
Escobar, C
中科院分区：
文献类型：
--
作者：
Angeles-Castellanos, M;Mendoza, J;Escobar, C
当每天的食物限制在几个小时内时，动物会在获得食物前2-3小时增加运动活动，这被称为食物预期活动。食物夹带与昼夜节律食物夹带振荡器（FEO）的表达有关，该振荡器的解剖底物似乎依赖于多种神经系统和外周器官。以前，我们已经描述了下丘脑核在食物携带过程中的不同参与。对于食物夹带通路，胃肠道系统和中枢神经系统之间的交流是必不可少的。脑干的内脏突触输入到达迷走神经背侧复合体，并直接从孤立束核（NST）或经臂旁核（PBN）传递到下丘脑核和前脑的其他区域。本研究旨在描述食物夹带时脑干结构的反应。c-Fos免疫反应性（c-Fos- ir）的表达被用来识别神经元的激活。目前的数据显示，在研究的所有脑干核中，用餐时间后c-Fos-IR增加。食物携带的时间模式在禁食条件下不会持续存在，这表明这种激活直接依赖于进食引发的信号。由于NST和PBN表现出与正常饮食后不同且增加的反应，我们认为食物干扰促进了摄入适应，从而导致这些脑干核的激活改变，例如胃膨胀。这些核向大脑提供的神经信息可能为FEO提供必要的牵引信号。
When food is restricted to a few hours daily, animals increase their locomotor activity 2-3 h before food access, which has been termed food anticipatory activity. Food entrainment has been linked to the expression of a circadian food-entrained oscillator (FEO) and the anatomic substrate of this oscillator seems to depend on diverse neural systems and peripheral organs. Previously, we have described a differential involvement of hypothalamic nuclei in the food-entrained process. For the food entrainment pathway, the communication between the gastrointestinal system and central nervous system is essential. The visceral synaptic input to the brain stem arrives at the dorsal vagal complex and is transmitted directly from the nucleus of the solitary tract (NST) or via the parabrachial nucleus (PBN) to hypothalamic nuclei and other areas of the forebrain. The present study aims to characterize the response of brain stem structures in food entrainment. The expression of c-Fos immunoreactivity (c-Fos-IR) was used to identify neuronal activation. Present data show an increased c-Fos-IR following meal time in all brain stem nuclei studied. Food-entrained temporal patterns did not persist under fasting conditions, indicating a direct dependence on feeding-elicited signals for this activation. Because NST and PBN exhibited a different and increased response from that expected after a regular meal, we suggest that food entrainment promotes ingestive adaptations that lead to a modified activation in these brain stem nuclei, e. g., stomach distension. Neural information provided by these nuclei to the brain may provide the essential entraining signal for FEO.