Escherichia coli O121:H19 infection identified on microagglutination assay and PCR.
Escherichia coli O121:H19 infection identified on microagglutination assay and PCR.
复制标题
通过微量凝集试验和 PCR 鉴定出大肠杆菌 O121:H19 感染。
DOI：
10.1111/ped.12699
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Takeuchi Y.
中科院分区：
文献类型：
--
作者：
Sakai T;Sawai T;Shimizu Y;Morimune T;Okuda Y;Maruo Y;Iyoda S;Takeuchi Y.
非O157产志贺毒素大肠杆菌（STEC）菌株越来越被认为是引发溶血性尿毒症综合征（HUS）的食源性病原体。这些菌株的检测和分离是重要的，但区分它们的细菌学特征是困难的。一名2岁女童在食用烤肉22天后发生溶血性尿毒综合征，伴轻度肾脏受累。未经特殊治疗，临床和实验室结果逐渐改善。因为两者都没有肠出血。在临床实验室的粪便培养中检测到大肠杆菌（EHEC）和志贺毒素，并且患者O157脂多糖循环抗体检测为阴性，该病例最初被诊断为可能的非典型溶血性尿毒综合征。然而，随后的血清诊断微凝集试验和基于聚合酶链反应的分子检测表明，存在携带stx2的EHEC O121:H19菌株。因此，为了正确诊断和治疗溶血性尿毒综合征，迫切需要在临床环境中检测非O157产志贺毒素大肠杆菌的系统。
Non‐O157 Shiga toxin‐producingEscherichia coli(STEC) strains are increasingly recognized as foodborne pathogens that trigger hemolytic uremic syndrome (HUS). The detection and isolation of these strains is important, but distinguishing their bacteriological profiles is difficult. A 2‐year‐old girl developed HUS with mild renal involvement 22 days after consuming barbecued meat. Clinical and laboratory findings gradually improved without specific treatment. Because neither enterohemorrhagicE.coli(EHEC) nor Shiga toxins were detected in stool cultures in a clinical laboratory and the patient tested negative for circulating antibodies to O157 lipopolysaccharide, the case was initially diagnosed as probable atypical HUS. Subsequent serodiagnostic microagglutination assay and polymerase chain reaction‐based molecular testing, however, indicated the presence of the EHEC O121:H19 strain withstx2. Thus, to correctly diagnose and treat HUS, a system for detecting non‐O157 STEC in a clinical setting is urgently needed.