Human muscle satellite cells as targets of Chikungunya virus infection.
Human muscle satellite cells as targets of Chikungunya virus infection.
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DOI：
10.1371/journal.pone.0000527
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发表时间：
2007-06-13
期刊：
影响因子：
3.7
通讯作者：
Ceccaldi PE
中科院分区：
文献类型：
--
作者：
Ozden S;Huerre M;Riviere JP;Coffey LL;Afonso PV;Mouly V;de Monredon J;Roger JC;El Amrani M;Yvin JL;Jaffar MC;Frenkiel MP;Sourisseau M;Schwartz O;Butler-Browne G;Desprès P;Gessain A;Ceccaldi PE
基孔肯雅(Chik)病毒是一种蚊子传播的甲型病毒，可在人类中引起以发烧、多关节痛、头痛和肌肉痛为特征的急性感染。自2005年以来，Chik病毒的出现与印度洋史无前例的Chik病暴发有关。在临床上，这次暴发的特点是多关节痛无效，97.7%的病例报告有肌痛。由于Chik病毒在人类体内的细胞靶点尚不清楚，我们研究了Chik病毒在骨骼肌中的致病事件和靶点。对两例Chik病毒感染的肌炎综合征患者的肌肉活检的免疫组织学研究表明，病毒抗原仅存在于骨骼肌祖细胞(设计为卫星细胞)中，而不是在肌肉纤维中。为了评估Chik病毒在人类卫星细胞中复制的能力，我们评估了病毒对原代人类肌肉细胞的感染；在Chik病毒感染的卫星细胞中观察到病毒生长并产生细胞病变效应，而肌管基本上对感染具有抵抗力。这份报告对Chik病毒的发病机制提供了新的见解，因为它是第一个在人类中发现Chik病毒的细胞靶点，并报告了一种病毒制剂选择性感染人类肌肉卫星细胞的报告。
Chikungunya (CHIK) virus is a mosquito-transmitted alphavirus that causes in humans an acute infection characterised by fever, polyarthralgia, head-ache, and myalgia. Since 2005, the emergence of CHIK virus was associated with an unprecedented magnitude outbreak of CHIK disease in the Indian Ocean. Clinically, this outbreak was characterized by invalidating poly-arthralgia, with myalgia being reported in 97.7% of cases. Since the cellular targets of CHIK virus in humans are unknown, we studied the pathogenic events and targets of CHIK infection in skeletal muscle. Immunohistology on muscle biopsies from two CHIK virus-infected patients with myositic syndrome showed that viral antigens were found exclusively inside skeletal muscle progenitor cells (designed as satelllite cells), and not in muscle fibers. To evaluate the ability of CHIK virus to replicate in human satellite cells, we assessed virus infection on primary human muscle cells; viral growth was observed in CHIK virus-infected satellite cells with a cytopathic effect, whereas myotubes were essentially refractory to infection. This report provides new insights into CHIK virus pathogenesis, since it is the first to identify a cellular target of CHIK virus in humans and to report a selective infection of muscle satellite cells by a viral agent in humans.
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影响因子：
7.7
作者：
Navarro-Sanchez, E;Altmeyer, R;Desprès, P
通讯作者：
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通讯作者：
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作者：
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作者：
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