Identification of proteins associated with cytoplasmic male sterility in pepper (Capsicum annuum L.)
Identification of proteins associated with cytoplasmic male sterility in pepper (Capsicum annuum L.)
复制标题
DOI：
10.1016/j.sajb.2015.04.010
复制
发表时间：
2015-09
影响因子：
3.1
通讯作者：
X. F. Zhang;B. Chen;L. Zhang;L. L. Zhang-L.;X. H. Chen;H. Zhao;S. Geng
中科院分区：
文献类型：
--
作者：
X. F. Zhang;B. Chen;L. Zhang;L. L. Zhang-L.;X. H. Chen;H. Zhao;S. Geng
辣椒(Capsicum annuumL.)是一种全球重要且经常异花授粉的蔬菜，表现出显著的杂种优势。尽管细胞质雄性不育(CMS)对辣椒杂交制种具有重要价值，但其分子机制尚不清楚。为了从蛋白质组水平上探讨辣椒细胞质雄性不育的分子机制，本研究对辣椒细胞质雄性不育系FS1030A及其保持系FS1030B的小孢子母细胞败育过程中的花药蛋白质组进行了观察。采用双向差示凝胶电泳法(2D-DGE)和液质联用法(LC-MS/MS)分析差异表达蛋白质。在每个凝胶上检测到约1070个花药蛋白质点，其中13个蛋白质点在FS1030A和FS1030B上有差异表达(p<0.05)。在13个蛋白质斑点中，有12个(92.3%)被NCBInr成功地鉴定为9个蛋白质，即下调的磷酸丙糖异构酶、肌动蛋白、谷胱甘肽S转移酶、铜锌超氧化物歧化酶、上调的支链α-酮酸脱氢酶E3亚基、酮酸还原异构酶、天冬氨酸蛋白酶和新生多肽。参与糖酵解的磷酸丙糖异构酶的低表达显著影响氧化磷酸化，最终导致ATP生成减少，而天冬氨酸蛋白酶的过度表达阻止正常的程序性细胞死亡(PCD)在CMS-FS1030A的发生中起着重要作用。这是首次利用蛋白质组学方法对辣椒细胞质雄性不育进行研究。这些结果为辣椒细胞质雄性不育的进一步研究奠定了基础。
Pepper (Capsicum annuumL.) is a globally important and often cross-pollinated vegetable that exhibits significant heterosis. Though cytoplasmic male sterility (CMS) has an important value for pepper hybrid production, its molecular mechanism remains unclear. To explore the molecular mechanism of CMS in pepper at the proteome level, we examined the anther proteomes of a cytoplasmic male sterile line FS1030A and its maintainer line FS1030B by transmission electronic microscopy (TEM) during abortion at the microspore mother cell stage. Two dimension difference gel electrophoresis (2D-DIGE) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) analyses were used to determine differentially expressed proteins. About 1070 anther protein spots were detected on each gel, and 13 protein spots were expressed differently between FS1030A and FS1030B (p < 0.05). Twelve of the thirteen protein spots (92.3%) were successfully identified by NCBInr as nine proteins, namely down-regulated triosephosphate isomerase, actin, glutathione S-transferase (GST), Cu/Zn superoxide dismutase (Cu/Zn-SOD), and up-regulated branched-chain α-keto acid dehydrogenase E3 subunit, ketol-acid reductoisomerase (KARI), aspartic protease, and nascent polypeptides. The low-expression of triosephosphate isomerase involved in glycolysis impacts oxidative phosphorylation significantly and ultimately results in a reduction in ATP production, and over-expression of aspartic protease blocking normal programmed cell death (PCD) played important roles in the occurrence of CMS-FS1030A. This is the first study on pepper CMS using a proteomic approach. These findings provide a good basis for further studies of CMS in pepper.