Field-Effect Enzymatic Amplifying Detector with Picomolar Detection Limit
Field-Effect Enzymatic Amplifying Detector with Picomolar Detection Limit
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DOI：
10.1021/ac901126d
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发表时间：
2009-08-15
影响因子：
7.4
通讯作者：
Yau, Siu-Tung
中科院分区：
文献类型：
--
作者：
Choi, Yongki;Yau, Siu-Tung
已经证明了酶检测器的输出电流的电压控制放大。通过在门控电极和其上固定有酶葡萄糖氧化酶的工作电极之间施加外部电压，检测器的生物催化输出电流显著增加，使得葡萄糖的检测限从毫摩尔水平降低到皮摩尔水平。电流放大可以通过施加的电压可逆地控制。将该技术应用于乙醇-乙醇脱氢酶系统显示出类似的结果。检测设置表明，输出电流由溶液和工作电极之间的界面处的电场控制。酶的生物特异性在磁场存在下得以保留。检测器的输出电流由施加在第三电极上的电压控制，其表现为场效应晶体管，其电流产生机制是使用酶作为催化剂将分析物转化为产物。在更广泛的意义上，检测器的操作显示了用于操纵氧化还原酶促反应的手段。
Voltage-controlled amplification of the output current of an enzymatic detector has been demonstrated. By application of an external voltage between the gating electrode and the working electrode on which the enzyme glucose oxidase was immobilized, the biocatalytic output current of the detector was increased significantly, allowing the detection limit of glucose to be lowered from the millimolar level to the picomolar level. The current amplification could be reversibly controlled by the applied voltage. Application of this technique to the ethanol-alcohol dehydrogenase system showed similar results. The detection setup suggests that the output current is controlled by the electric field at the interface between the solution and the working electrode. The enzyme's biospecificity was preserved in the presence of the field. The detector, with its output current controlled by a voltage applied at a third electrode, behaves as a field-effect transistor, whose current-generating mechanism is the conversion of an analyte to a product using an enzyme as catalyst. In a broader sense, the operation of the detector shows a means for manipulating a redox enzymatic reaction.