Ciliary metachronal wave propagation on the compliant surface of Paramecium cells
Ciliary metachronal wave propagation on the compliant surface of Paramecium cells
复制标题
草履虫细胞顺应表面上的睫状异时波传播
作者：
Narematsu;N.;Quek;R.;Chiam;K.-H. and Iwadate;Y.
原生动物中的纤毛运动表现出异时性波状协调，其中相邻纤毛之间保持恒定的相位差。目前普遍认为，异时波需要相邻纤毛和细胞外液之间的流体动力学耦合。为了验证这一假设，我们使用完全密封细胞表面的微量移液器抽吸草履虫细胞，使得没有液体可以通过微量移液器。因此，细胞的前部和后部区域是流体动力学解耦的。尽管如此，我们仍然观察到异时波继续从细胞的前端向后端传播，这表明除了流体动力学耦合之外，还有其他机制也可以传播异时波。在计算建模中也观察到这种传输，其中流体在跳动纤毛阵列的两个分区之间完全解耦。我们还对草履虫细胞表面进行了周期性拉伸，发现在周期性拉伸的存在下，异时波仍然存在。这表明，除了流体动力学耦合，顺应性基板也可以发挥重要作用，调解异时波的传播。© 2015 Wiley Periodicals，Inc.
Ciliary movements in protozoa exhibit metachronal wave‐like coordination, in which a constant phase difference is maintained between adjacent cilia. It is at present generally thought that metachronal waves require hydrodynamic coupling between adjacent cilia and the extracellular fluid. To test this hypothesis, we aspirated aParameciumcell using a micropipette which completely sealed the surface of the cell such that no fluid could pass through the micropipette. Thus, the anterior and the posterior regions of the cell were hydrodynamically decoupled. Nevertheless, we still observed that metachronal waves continued to propagate from the anterior to the posterior ends of the cell, suggesting that in addition to hydrodynamic coupling, there are other mechanisms that can also transmit the metachronal waves. Such transmission was also observed in computational modeling where the fluid was fully decoupled between two partitions of a beating ciliary array. We also imposed cyclic stretching on the surface of liveParameciumcells and found that metachronal waves persisted in the presence of cyclic stretching. This demonstrated that, in addition to hydrodynamic coupling, a compliant substrate can also play a critical role in mediating the propagation of metachronal waves. © 2015 Wiley Periodicals, Inc.