Seasonal profiles of human noroviruses and indicator bacteria in a wastewater treatment plant in Tokyo, Japan
Seasonal profiles of human noroviruses and indicator bacteria in a wastewater treatment plant in Tokyo, Japan
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DOI：
10.2166/wst.2006.888
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发表时间：
2006-01-01
影响因子：
2.7
通讯作者：
Ohgaki, S.
中科院分区：
文献类型：
--
作者：
Haramoto, E.;Katayama, H.;Ohgaki, S.
从2003年7月至2004年6月，在日本东京的一个污水处理厂，每月定量测定了原污水，二次处理污水和最终出水中微生物的季节性分布。用实时荧光定量PCR方法检测的人诺如病毒基因1型在0.17- 260拷贝/mL之间，基因2型在2.4- 1900拷贝/mL之间，在流行季节冬季的数值更高。总大肠菌群，大肠杆菌，或F-特异性大肠杆菌在原污水的浓度几乎是恒定的全年。在污水处理厂，基因型2的人类诺如病毒被最有效地去除（平均3.69 log（10）），其次是大肠杆菌（3.37 log（10）），总大肠菌群（3.05 log（10）），F特异性大肠杆菌（2.81 log（10））和基因型1的人类诺如病毒（2.27 log（10））。人诺如病毒的去除率几乎是恒定的，独立于原污水中的病毒的初始浓度，这导致了在冬季的最终出水中的人诺如病毒的浓度增加。没有一种测试细菌被判定为最终流出物中人类诺如病毒的可靠指示剂。
The seasonal profiles of microorganisms in raw sewage, secondary-treated sewage, and final effluent at a wastewater treatment plant in Tokyo, Japan, were quantitatively determined each month for one year, from July 2003 to June 2004. Human noroviruses, which were determined by real-time PCR, in raw sewage varied from 0.17- 260 copies/mL for genotype 1 and from 2.4- 1900 copies/mL for genotype 2, showing much higher values in winter, the epidemic season. The concentration of total coliforms, Escherichia coli, or F-specific phages in raw sewage was almost constant throughout the year. Human noroviruses of genotype 2 were removed most effectively (3.69 log(10), on average) at the wastewater treatment plant, followed by E coli (3.37 log(10)), total coliforms (3.05 log(10)), F-specific phages (2.81 log(10)), and human noroviruses of genotype 1 (2.27 log(10)). The removal ratio of human norovinuses was almost constant, independent of the initial concentration of the viruses in raw sewage, which led to the increasing concentration of human noroviruses in final effluent in winter. None of the tested bacteria was judged to be a reliable indicator of human noroviruses in final effluent.