TPS46, a Rice Terpene Synthase Conferring Natural Resistance to Bird Cherry-Oat Aphid, Rhopalosiphum padi (Linnaeus).
TPS46, a Rice Terpene Synthase Conferring Natural Resistance to Bird Cherry-Oat Aphid, Rhopalosiphum padi (Linnaeus).
复制标题
DOI：
10.3389/fpls.2017.00110
复制
发表时间：
2017
影响因子：
5.6
通讯作者：
Guo Y
中科院分区：
文献类型：
--
作者：
Sun Y;Huang X;Ning Y;Jing W;Bruce TJ;Qi F;Xu Q;Wu K;Zhang Y;Guo Y
植物萜烯脱氢酶（TPS）是萜烯生物合成的关键酶，在防御害虫侵害中发挥重要作用。在水稻中，TPS 46的蛋白质序列与玉米TPS 10最接近。然而，与玉米tps 10不同，tps 46也在水稻中组成型表达，即使在没有草食动物攻击的情况下。特定挥发物的潜在作用或组成性排放可能归因于水稻中tps 46的组成性表达。因此，在本研究中，RNA干扰（Ri）和过表达（Oe）水稻品系，研究TPS 46在水稻中的潜在功能。有趣的是，与Wt相比，在温室条件下，当tps 46的表达被沉默时，水稻植株变得对禾谷缢管蚜更敏感。人工侵染生物测定进一步证实了Ri系对稻曲病的感病性。padi，而Oe系对R.帕迪。基于GC-MS和ToF-MS分析，共鉴定出8种TPS 46催化的挥发性产物。其中，在Ri、Oe和Wt品系中均检测到柠檬烯和Eβf，而其他6种挥发物仅在Oe品系的混合物中检测到。Ri系的组成型柠檬烯和Eβf含量显著低于Wt系，而Oe系的这两种挥发物含量显著高于对照。这些结果表明，由tps 46组成型表达调控的Eβf和柠檬烯组成型释放可能在水稻抗赤霉病中起重要作用。帕迪。因此，tps 46可能是一个潜在的目的基因，用于提高植物对蚜虫的抗性。
Plant terpene synthases (TPSs) are key enzymes responsible for terpene biosynthesis, and can play important roles in defense against herbivore attack. In rice, the protein sequence of TPS46 was most closely related to maize TPS10. However, unlike maize tps10, tps46 was also constitutively expressed in rice even in the absence of herbivore attack. Potential roles or constitutive emissions of specific volatiles may due to the constitutive expressions of tps46 in rice. Therefore, in the present study, RNA interference (Ri) and overexpression (Oe) rice lines were generated to investigate the potential function of TPS46 in Oryza sativa sp. japonica. Interestingly, the rice plants become more susceptible to Rhopalosiphum padi when expression of tps46 was silenced compared with Wt in greenhouse conditions. Artificial infestation bioassays further confirmed that Ri rice lines were susceptible to R. padi, whereas Oe rice lines were repellent to R. padi. Based on GC-MS and ToF-MS analysis, a total of eight volatile products catalyzed by TPS46 in rice were identified. Among them, only limonene and Eβf could be detected in all the Ri, Oe, and Wt lines, whereas other six volatiles were only found in the blend of volatiles from Oe lines. Moreover, the amount of constitutive limonene and Eβf in the Ri lines was significantly lower than in Wt lines, while the amounts of these two volatiles in the Oe line were obviously higher than in control rice. Our data suggested that the constitutive emissions of Eβf and limonene regulated by the constitutive expression of tps46 may play a crucial role in rice defense against R. padi. Consequently, tps46 could be a potential target gene to be employed for improving the resistance of plants to aphids.