X-ray-sensitive mutants of mouse mammary carcinoma cells are hypersensitive to bleomycin and hydrogen peroxide.
X-ray-sensitive mutants of mouse mammary carcinoma cells are hypersensitive to bleomycin and hydrogen peroxide.
复制标题
小鼠乳腺癌细胞的 X 射线敏感突变体对博莱霉素和过氧化氢过敏。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
T. Nakazawa
中科院分区：
文献类型：
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作者：
K. Sato;A. Ito;T. Shiomi;H. Hama;H. Ishikawa;T. Yoshizumi;T. Nakazawa
从小鼠乳腺癌FM 3A细胞中分离出辐射敏感突变体SX 9和SX 10。突变体SX 9与另一种小鼠白血病L5178 Y细胞的辐射敏感株M10互补，但SX 10没有。SX 9和SX 10细胞比野生型细胞对博莱霉素的致死作用更敏感。在高达1戈伊的剂量范围内，SX 9细胞中对6-硫代鸟嘌呤抗性的X射线诱导突变的频率远高于野生型细胞，尽管当在等效存活率下比较时，这两种细胞株之间诱导突变的频率没有很大差异。发现SX 9、SX 10和M10细胞对过氧化氢失活的敏感性远高于相应的野生型细胞，但这些细胞株的过氧化氢酶活性水平相似。这些突变体对于克隆和鉴定负责辐射敏感性的人类基因是有用的。
Radiation-sensitive mutants, SX9 and SX10, were isolated from mouse mammary carcinoma FM3A cells. The mutant SX9 complemented another radiation-sensitive strain M10 of mouse leukemia L5178Y cells, but SX10 did not. SX9 and SX10 cells were more sensitive than the wild-type cells to the lethal effects of bleomycin. The frequency of X-ray-induced mutations to 6-thioguanine resistance was much higher in SX9 cells than in the wild-type cells in the dose range up to 1 Gy, although the induced mutant frequencies were not very different between these two cell strains when compared at equivalent survival. SX9, SX10 and M10 cells were found to be far more sensitive than the respective wild-type cells to hydrogen peroxide inactivation, but the levels of catalase activity were similar among these cell strains. These mutants should be useful for cloning and identifying the human genes responsible for radiation sensitivity.