Developmental changes in pulp organic acid concentration and activities of acid-metabolising enzymes during the fruit development of two loquat (Eriobotrya japonica Lindl.) cultivars differing in fruit acidity
Developmental changes in pulp organic acid concentration and activities of acid-metabolising enzymes during the fruit development of two loquat (Eriobotrya japonica Lindl.) cultivars differing in fruit acidity
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DOI：
10.1016/j.foodchem.2008.10.003
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发表时间：
2009-05-15
期刊：
影响因子：
8.8
通讯作者：
Chen, Li-Song
中科院分区：
文献类型：
--
作者：
Chen, Fa-Xing;Liu, Xing-Hui;Chen, Li-Song
枇杷果实中有机酸含量及相关酶活性的变化以低酸‘长虹3号’和高酸‘解放钟’为试材，研究了不同品种的纸浆品质。可滴定酸（TA）和苹果酸浓度在果实发育的早期阶段增加，并在后期下降。两个品种TA的差异可以用苹果酸浓度的差异来解释，苹果酸浓度的差异可能是由NAD-苹果酸脱氢酶（NAD-MDH）和NADP-苹果酸酶（NADP-ME）活性的差异造成的。尽管两个品种苹果酸浓度的差异不是由磷酸烯醇式丙酮酸羧化酶（PEPC）活性的差异引起的，但“昌红3号”果实发育过程中的苹果酸浓度和“解放钟”早期的苹果酸浓度分别随着PEPC活性的增加而线性增加和曲线增加。因此，NAD-MDH。NADP-ME和PEPC活性在苹果酸的生物合成和降解过程中起重要作用。(c)2008爱思唯尔有限公司版权所有。
Changes in organic acid concentration and related enzyme activities in loquat (Eriobotrya japonica Lindl.) pulp were studied, using low-acid 'Changhong 3' and high-acid 'Jiefangzhong' cultivars. Both titratable acidity (TA) and malic acid concentration increased during the early stages of fruit development and decreased at the later stages. The difference in TA between the two cultivars could be explained by the difference in malic acid concentration, which could result from a difference in NAD-malate dehydrogenase (NAD-MDH) and NADP-malic enzyme (NADP-ME) activities. Although the difference in malic acid concentration between the two cultivars could not result from a difference in phosphoenolpyruvate carboxylase (PEPC) activity, malic acid concentration in both 'Changhong 3' throughout fruit development and 'Jiefangzhong' at the early stages increased linearly and curvilinearly with increasing PEPC activity, respectively. Therefore, NAD-MDH. NADP-ME and PEPC activities may play significant roles in malic acid biosynthesis and degradation. (c) 2008 Elsevier Ltd. All rights reserved.