Effects of simulated solar radiation on conidial germination of Metarhizium anisopliae in different formulations
Effects of simulated solar radiation on conidial germination of Metarhizium anisopliae in different formulations
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DOI：
10.1016/s0261-2194(98)00074-x
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发表时间：
1998-11-01
期刊：
影响因子：
2.8
通讯作者：
Leather, SR
中科院分区：
文献类型：
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作者：
Alves, RT;Bateman, RP;Leather, SR
采用不同配方研究了模拟太阳辐射时间对金龟子绿僵菌分生孢子萌发的影响，以确定其中一些配方是否能提高分生孢子的紫外线耐受性。分生孢子悬浮液在水+10%乳化油、植物油、矿物油和水+0.05%吐温80中制备。将每毫升1×10(8)/毫升的等量分生孢子悬浮液(7.2MU L)放入培养皿中，分别在‘Oriel’日照模拟器上照射2、4和6h。所有配方的未曝光平板均为对照。照射后，用等体积的Shellsol T或0.05%吐温80稀释得到的悬浮液，在27+/-0.5℃的沙堡-右旋糖琼脂上孵育，24和48h后评估分生孢子的存活率。随着模拟太阳辐射暴露时间的增加，所有处理的发芽率都有所下降。花生油、贝壳素+Ondina、水+Emoleo(R)、Codacide(R)、Natur‘l Oil(R)、Ashlade(R)显著提高了分生孢子的耐受性(P
The effects of exposure time to simulated solar radiation on conidial germination of Metarhizium anisopliae were evaluated with different formulations to determine whether some of them could enhance the ultraviolet (UV) tolerance of conidia. Conidial suspensions were prepared in water plus 10% emulsifiable oils; vegetable oil; mineral oils; and in water plus 0.05% Tween 80. Equal volumes (7.2 mu l) of each 1 x 10(8)/ml conidial suspension were placed into Petri dishes, and exposed to 2, 4 and 6 h solar radiation from an 'Oriel' sunlight simulator. Unexposed plates of all formulations were the controls. After irradiation, the formulations were diluted with equal volumes of Shellsol T or 0.05% Tween 80, and the resultant suspensions incubated on Sabouraud-Dextrose-Agar at 27 +/- 0.5 degrees C. Conidial viability was assessed after 24 and 48 h incubation. Germination decreased for all treatments when the time of exposure to simulated solar radiation increased. Peanut oil, Shellsol plus Ondina, water plus Emoleo(R), Codacide(R), Natur'l oil(R), Ashlade(R) significantly enhanced conidial tolerance (P