PLAQUE ASSAY FOR TITRATION OF ALFALFA LOOPER NUCLEAR POLYHEDROSIS-VIRUS IN A CABBAGE-LOOPER (TN-368) CELL LINE
PLAQUE ASSAY FOR TITRATION OF ALFALFA LOOPER NUCLEAR POLYHEDROSIS-VIRUS IN A CABBAGE-LOOPER (TN-368) CELL LINE
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DOI：
10.1016/0022-2011(73)90129-8
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发表时间：
1973-01-01
影响因子：
3.4
通讯作者：
VAIL, PV
中科院分区：
文献类型：
--
作者：
HINK, WF;VAIL, PV
这是首次报道了一种致病昆虫病毒的空斑形成。用0.6%甲基纤维素包被的TN-368细胞在感染紫花苜蓿环状核型多角体病毒后继续增殖，发育成单层，并产生空斑。病毒暴露细胞24小时后首次观察到病毒多角体包涵体，72小时后斑块继续增大。观察到两种不同类型的斑块：一种是所有细胞的细胞核中都有许多多面体，另一种是细胞很少有包涵体。当两个斑块中的病毒被注入体内时。Nilarvae，他们死于典型的核多角体病毒病。该方法重复性好，斑块数量与病毒浓度有关。
This is the first report of plaque formation by a pathogenic insect virus.Trichoplusia ni(TN-368) cells overlaid with medium containing 0.6% methyl cellulose continued to multiply, developed into monolayers, and produced plaques after infection with alfalfa looper nuclear polyhedrosis virus. Viral polyhedral inclusion bodies were first observed 24 hr after exposure of cells to virus, and plaques continued to increase in size for 72 hr. Two different types of plaques were observed: one in which all cells had many polyhedra in their nuclei, and another in which few cells had inclusion bodies. When virus from either plaque was injected intoT. nilarvae, they died of typical nuclear polyhedrosis virus disease. The assay was reproducible, and plaque numbers were related to virus concentration.