The ribonuclease/angiogenin inhibitor is also present in mitochondria and nuclei
The ribonuclease/angiogenin inhibitor is also present in mitochondria and nuclei
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DOI：
10.1016/j.febslet.2011.01.034
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发表时间：
2011-02-18
期刊：
影响因子：
3.5
通讯作者：
D'Alessio, Giuseppe
中科院分区：
文献类型：
--
作者：
Furia, Adriana;Moscato, Marco;D'Alessio, Giuseppe
本文提供的数据首次表明，被称为“核糖核酸酶（RNase）抑制剂”（RI或RNH 1）的蛋白质不仅存在于细胞胞质溶胶中，而且存在于线粒体中，线粒体是细胞氧化还原稳态的中心细胞器。这一发现与报道的RI保护细胞免受氧化应激的能力直接相关，其对氧化的敏感性和作为活性氧清除剂的反应性。在进行这项研究的同时，我们还惊奇地发现RI在细胞核中的存在。我们认为这些数据在研究RI的细胞作用方面开辟了新的观点。结构化总结：RI与ATP合成酶亚α线粒体、应激70蛋白、线粒体、钙结合线粒体载体蛋白Arrhythm 2 ADP/ATP转位酶2延伸因子Tu和细胞色素b-c1复合物亚2通过抗诱饵共免疫沉淀（查看相互作用）PARP和RI通过共沉淀共定位（查看相互作用）（C）2011欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The data presented here show for the first time that the protein known as "ribonuclease (RNase) inhibitor" (RI or RNH1) is present not only in the cell cytosol, but also in mitochondria, the central organelles in cell redox homeostasis. This finding directly correlates with the reported ability of RI to protect the cell from oxidative stress, with its sensitivity to oxidation and reactivity as a reactive oxygen species scavenger. While this study was carried out we also surprisingly discovered the presence of RI in the cell nucleus. We deem that these data open new views in the investigation on the cellular role(s) of the RI.Structured summary:RI physically interacts with ATP synthase sub Alpha mitochondrial, Stress 70 Protein, Mitochondrial, Calcium-binding mitochondrial carrier protein Aralar 2 ADP/ATP Translocase 2 Elongation factor Tu and Cytochrome b-c1 complex Sub 2 by anti bait coimmunoprecipitation (View interaction) PARP and RI colocalize by cosedimentation (View interaction) (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.